ZFIN ID: ZDB-FIG-140214-17
Dheen et al., 1999 - Zebrafish tbx-c functions during formation of midline structures. Development (Cambridge, England)   126(12):2703-2713
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to 20-25 somites

Fig. 3 Expression pattern of tbx-c, tbx-b and tbx-a in the tail and discrete cell groups in the chordoneural hinge (CNH) of zebrafish at 18-20 hpf. (A) tbx-c is expressed in the RB cells, the excretory system and the CNH (outlined). (B) tbx-b is expressed in the notochord. (C) tbx-a is expressed in the excretory system. (D) Double staining for Ntl protein (brown) and tbx-c mRNA (blue). Ntl is expressed in the posterior mesoderm, the CNH and the notochord. tbx-c transcripts map to the anterior CNH. (E) shh is expressed in the CNH, the notochord and the floor plate. (F) Two-colour in situ hybridization staining for tbx-c (blue) and flh (magenta). tbx-c is expressed in the anterior CNH, whereas flh is expressed in the posterior part of the CNH. Expression domains of flh and tbx-c overlap in the mid-CNH (arrows). (G) tbx-c is expressed in the anterior part of the CNH. (H) A model summarizing the distribution of gene products in the CNH and the notochord. cnh, chordoneural hinge; ex, excretory system; fp, floor plate; n, notochord; pm, posterior mesoderm. Scale bars, 25 μm

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
noto WT standard conditions 14-19 somites to 20-25 somites chordo neural hinge medial region ISH
14-19 somites to 20-25 somites chordo neural hinge posterior region ISH
shha WT standard conditions 14-19 somites to 20-25 somites chordo neural hinge ISH
14-19 somites to 20-25 somites floor plate ISH
14-19 somites to 20-25 somites notochord ISH
tbx2a WT standard conditions 14-19 somites to 20-25 somites cloacal chamber ISH
tbx2b WT standard conditions 14-19 somites to 20-25 somites chordo neural hinge anterior region ISH
14-19 somites to 20-25 somites chordo neural hinge medial region ISH
14-19 somites to 20-25 somites Rohon-Beard neuron ISH
tbx3a WT standard conditions 14-19 somites to 20-25 somites notochord ISH
tbxta Ab1-ta WT standard conditions 14-19 somites to 20-25 somites chordo neural hinge IHC
Ab1-ta 14-19 somites to 20-25 somites notochord IHC
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.