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ZFIN ID: ZDB-FIG-140214-26
Dheen et al., 1999 - Zebrafish tbx-c functions during formation of midline structures. Development (Cambridge, England)   126(12):2703-2713
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: 1-4 somites to 10-13 somites
PHENOTYPE:
Fish:
Observed In:
Stage Range: 1-4 somites to 10-13 somites

Fig. 6 Analysis of tbx-c expression and effect of ectopic expression of tbx-c+ in mutants. (A,B) Expression of tbx-c in wild-type (A) and ntl-/- mutant (B) embryos. Note the segregation of axial mesoderm (arrowheads) and a low level expression of tbx-c in the midline at the trunk of the mutant (arrows). (C-E) Co-localization of ehh (blue) and Ntl (brown) in wild-type (C), tbx-c+ injected ntl-/- (D) and normal sibling(E) embryos obtained by crossing heterozygous flh-/+ pair. Expression of both ehh and Ntl is expanded in the tbx-c+ injected wild-type sibling embryo (E) and absent in the tbx-c+ injected ntl-/- mutant embryo (D). (F) tbx-c expression in the flh-/- embryo. The notochord is absent and tbx-c is undetectable in the midline region of flh-/-embryo. (G,H) ntl expression in the flh-/- embryo (G) and the flh-/- embryo injected with tbx-c+ (H). A few ntl positive cells (arrows) can be seen in the midline of both group of embryos. (I,J) tbx-c expression in wild-type (I) and spt-/- embryos (J) at 14 hpf. Arrows indicate the tbx-c expression in the notochord. Insert (C,E): high magnification to show co-expression of ehh and Ntl in the notochord. Scale bars, 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
ihhb WT standard conditions 1-4 somites notochord ISH
tbx2b WT standard conditions 1-4 somites axial mesoderm ISH
noton1/n1 standard conditions 1-4 somites Not Detected axial mesoderm ISH
tbx16b104/b104 standard conditions 10-13 somites axial mesoderm ISH
tbxtab160/b160 standard conditions 1-4 somites axial mesoderm ISH
tbxta Ab1-ta WT standard conditions 1-4 somites axial mesoderm IHC
Ab1-ta 1-4 somites notochord IHC
noton1/n1 standard conditions 1-4 somites axial mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
noton1/n1 standard conditions 1-4 somites notochord absent, abnormal
tbx16b104/b104 standard conditions 10-13 somites notochord increased width, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.