FIGURE

Fig. 1

ID

ZDB-FIG-140122-21

Publication

Venkiteswaran et al., 2013 - Generation and Dynamics of an Endogenous, Self-Generated Signaling Gradient across a Migrating Tissue

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Fig. 1

Expression and Requirement of Sdf1a and Its Receptors Cxcr4b, Cxcr7a, and Cxcr7b during Primordium Migration

(A) Live images of embryos of the indicated stage and genotype. Arrow indicates the primordium, and arrowheads indicate neuromasts.

(B) Fluorescent staining for cxcr4b, cxcr7a, or cxcr7b mRNA and GFP protein at 36 hpf. Scale bar, 50 μm.

(C) Fluorescent staining for sdf1a mRNA and GFP protein in a tg(cldnB:lyn₂GFP) embryo at 36 hpf. Anterior is to the left, and posterior is to the right.

(D) Quantification of primordium migration in 48 hpf embryos of indicated genotypes. The vertical bars represent the average position of the primordium, the error bars represent SD, and the circles represent the positions of individual primordia. 48 hpf embryo schematic adapted from (Kimmel et al., 1995).

See also Figure S6.

Expression Data

Genes:	ackr3a ackr3b cxcl12a cxcr4b EGFP
Fish:	zf106Tg zf106Tg + MO1-ackr3a + MO2-ackr3a ackr3b^sa16/sa16; zf106Tg cxcl12a^{t30516/t30516}; zf106Tg cxcr4b^{t26035/t26035}; zf106Tg ackr3b^sa16/sa16; zf106Tg + MO1-ackr3a + MO2-ackr3a
Knockdown Reagents:	MO1-ackr3a MO2-ackr3a
Anatomical Terms:	posterior lateral line primordium posterior lateral line primordium anterior-most region
Stage Range:	Prim-5 to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zf106Tg + MO1-ackr3a + MO2-ackr3a ackr3b^sa16/sa16; zf106Tg cxcl12a^{t30516/t30516}; zf106Tg cxcr4b^{t26035/t26035}; zf106Tg ackr3b^sa16/sa16; sk78Tg; zf106Tg ackr3b^sa16/sa16; zf106Tg + MO1-ackr3a + MO2-ackr3a ackr3b^sa16/sa16; sk78Tg; zf106Tg + MO1-ackr3a + MO2-ackr3a
Knockdown Reagents:	MO1-ackr3a MO2-ackr3a
Observed In:	posterior lateral line neuromast primordium migration
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Cell, 155(3), Venkiteswaran, G., Lewellis, S.W., Wang, J., Reynolds, E., Nicholson, C., and Knaut, H., Generation and Dynamics of an Endogenous, Self-Generated Signaling Gradient across a Migrating Tissue, 674-687, Copyright (2013) with permission from Elsevier. Full text @ Cell