Fig. S5
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- ZDB-FIG-140122-26
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- Venkiteswaran et al., 2013 - Generation and Dynamics of an Endogenous, Self-Generated Signaling Gradient across a Migrating Tissue
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Ectopic Sources of Sdf1a Attract the Primordium, Related to Figure 3 (A) Heat-shocked tg(cldnB:lyn2GFP) embryo stained for GFP protein (brown) to visualize the primordium (arrow). (B) Similarly treated tg(cldnB:lyn2GFP); tg(hsp70:sdf1a) embryo. (C) Quantification of primordia morphology in heat-shocked wild-type and tg(hsp70:sdf1a) embryos. (D) Experimental strategy for local mis-expression of Sdf1a during primordium migration. (E–L) 36 hpf chimeric embryos comprised of tg(hsp70:sdf1a) donor cells and tg(cldnB:lyn2GFP) host cells. Embryos were stained for GFP protein to visualize the primordium (arrow) and sdf1a mRNA to identify donor cells that overexpress sdf1a. (E and F) Sdf1a mis-expression in some of the front and rear cells of the primordium (arrowheads in F) disrupts its migration (arrow in F) compared to the primordium on the contralateral control side of the same embryo (arrow in E). (G and H) Sdf1a mis-expression in the rear cells of the primordium (arrowhead in H) stalls primordium migration (arrow in H) compared to the contralateral control side of the same embryo (arrow in G). (I and J) Sdf1a mis-expression along the migratory route (arrowheads in J) redirects the primordium to the ectopic Sdf1a source (arrows in J) compared to the contralateral control side of the same embryo (I). (K and L) Sdf1a mis-expression in the somites results in a fragmented primordium (arrows in K and L) on both sides of the embryo. |
Reprinted from Cell, 155(3), Venkiteswaran, G., Lewellis, S.W., Wang, J., Reynolds, E., Nicholson, C., and Knaut, H., Generation and Dynamics of an Endogenous, Self-Generated Signaling Gradient across a Migrating Tissue, 674-687, Copyright (2013) with permission from Elsevier. Full text @ Cell