ZFIN ID: ZDB-FIG-131213-29
Schumacher et al., 2013 - tal1 regulates the formation of intercellular junctions and the maintenance of identity in the endocardium. Developmental Biology   383(2):214-226 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 14-19 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: 14-19 somites

Fig. 6 Endocardial intercellular junctions are disorganized during early endocardial morphogenesis in tal1-deficient embryos. (A–H) Transverse sections of wild-type (A, C, D, and G) and tal1-deficient embryos (B, E, F, and H), dorsal to the top, at 18s. Immunofluorescence detects localization of ZO-1 (red) (C–H) and F-actin (blue) (D and F); in addition, an anti-GFP antibody allows visualization of Tg(kdrl:GRCFP) expression (green) in the endocardium (A, B, G, and H). The boxed areas in (A and B) are highlighted in (C–H). (A) Wild-type endocardium forms a single-layered sheet with ZO-1 localized to discrete puncta at cell-cell contacts (C, D, and G). (B) tal1-deficient endocardium is multi-layered and disorganized, and ZO-1 is mislocalized in multiple puncta along the endocardial cell boundaries (E, F, and H). (G′ and H′) Cartoons indicate regions of ZO-1 (red) and F-actin (blue) localization within the endocardium.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
grcfp zn1Tg standard conditions 14-19 somites endocardium IHC
zn1Tg + MO5-tal1 + MO6-tal1 standard conditions 14-19 somites endocardium IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tjp1 IHC zn1Tg standard conditions 14-19 somites endocardium cell-cell junction
IHC zn1Tg + MO5-tal1 + MO6-tal1 standard conditions 14-19 somites endocardium cell-cell junction
Phenotype Details
Fish Conditions Stage Phenotype
zn1Tg + MO5-tal1 + MO6-tal1 standard conditions 14-19 somites endocardium cell-cell junction mislocalised, abnormal
14-19 somites endocardium cell-cell junction organization decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 383(2), Schumacher, J.A., Bloomekatz, J., Garavito-Aguilar, Z.V., and Yelon, D., tal1 regulates the formation of intercellular junctions and the maintenance of identity in the endocardium, 214-226, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.