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ZFIN ID: ZDB-FIG-131213-27
Schumacher et al., 2013 - tal1 regulates the formation of intercellular junctions and the maintenance of identity in the endocardium. Developmental Biology   383(2):214-226 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 10-13 somites to 14-19 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: 14-19 somites

Fig. 4 The architecture of the early endocardium is disrupted in tal1-deficient embryos. (A–J) Transverse sections of wild-type (A, C, E, G, and I) and tal1-deficient embryos (B, D, F, H, and J), dorsal to the top, at 16s (A and B) and 18s (C–J). Immunofluorescence detects localization of fibronectin (FN; green) (E–H) and β-catenin (red) (E, F, I, and J); in addition, an anti-GFP antibody allows visualization of Tg(kdrl:GRCFP) expression (blue) in the endocardium (A–F). Together, these markers highlight the shapes and arrangement of the endocardial cells. Endocardial cells form a single-layered sheet of cells at 16s (A), after they have migrated to the midline, and this sheet of cells is maintained at 18s (C). In tal1-deficient embryos, endocardial cells stack on top of each other, forming a multi-layered cluster (B and D). The boxed areas in (C and D) are highlighted in (E–J). The apical-basal polarity of wild-type endocardial cells (E) is indicated by basal deposition of FN (G) and apical enrichment of β-catenin (I). In tal1-deficient embryos (F), localization of FN (H) and β-catenin (J) highlight the dysmorphic endocardial architecture and aberrant cell shapes. (E′–J′) Cartoons indicate regions of FN (green) and β-catenin (red) localization within the endocardium. Endocardial cell outlines are marked in black. The FN and β-catenin that are presumed to be located within the endoderm that overlies the endocardium are excluded from the cartoons.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
grcfp zn1Tg standard conditions 14-19 somites endocardium IHC
zn1Tg + MO5-tal1 + MO6-tal1 standard conditions 10-13 somites endocardium IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-ctnnb IHC zn1Tg standard conditions 14-19 somites endocardium apical surface
IHC zn1Tg + MO5-tal1 + MO6-tal1 standard conditions 10-13 somites endocardium anatomical surface
Ab2-fn IHC zn1Tg standard conditions 14-19 somites endocardium basal surface
IHC zn1Tg + MO5-tal1 + MO6-tal1 standard conditions 10-13 somites endocardium anatomical surface
Phenotype Details
Fish Conditions Stage Phenotype
zn1Tg + MO5-tal1 + MO6-tal1 standard conditions 14-19 somites endocardium endothelial cell aggregated, abnormal
14-19 somites endocardium endothelial cell obtuse, abnormal
14-19 somites presumptive endocardium morphogenesis of an epithelial sheet decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 383(2), Schumacher, J.A., Bloomekatz, J., Garavito-Aguilar, Z.V., and Yelon, D., tal1 regulates the formation of intercellular junctions and the maintenance of identity in the endocardium, 214-226, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.