ZFIN ID: ZDB-FIG-131213-28
Schumacher et al., 2013 - tal1 regulates the formation of intercellular junctions and the maintenance of identity in the endocardium. Developmental Biology   383(2):214-226 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 5 Endocardial intercellular junctions are disorganized following heart tube extension in tal1-deficient embryos. (A–D) Immunofluorescence detects localization of ZO-1 (red) in wild-type (A and C) and tal1-deficient (B and D) hearts at 25 hpf; lateral views, ventricular end of the heart tube at the top. An anti-GFP antibody allows visualization of Tg(kdrl:GRCFP) expression (green) in the endocardium. (A and B) Three-dimensional reconstructions of confocal stacks demonstrate that ZO-1 is located around the perimeter of the myocardial cells in both wild-type and tal1-deficient hearts. (C) Optical slice of a wild-type heart reveals that ZO-1 is localized in discrete puncta at points of endocardial cell-cell contact; in addition, wild-type endocardial cells exhibit a flattened and elongated morphology. (D) Optical slice of a tal1-deficient heart reveals that ZO-1 is mislocalized in large clusters around the perimeter of the endocardial cells; in addition, the tal1-deficient endocardial cells exhibit a rounded shape, rather than the normal elongated shape. (E and F) Immunofluorescence detects ZO-1 (purple) in wild-type (E) and tal1-deficient (F) hearts at 25 hpf; lateral views, ventricular end of the heart tube at the top. Expression of the Tg(fli1a:negfp) (green) and Tg(myl7:H2A-mCherry) (red) transgenes reveals one endocardial cell with ectopic myocardial gene expression (arrow). Subcellular localization of ZO-1 is aberrant in the majority of tal1-deficient endocardial cells, nearly all of which do not display ectopic myocardial gene expression.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP sd12Tg; y7Tg(AB) standard conditions Prim-5 endocardium IHC
sd12Tg; y7Tg + MO5-tal1 + MO6-tal1(AB) standard conditions Prim-5 endocardium IHC
grcfp zn1Tg standard conditions Prim-5 endocardium IHC
zn1Tg + MO5-tal1 + MO6-tal1 standard conditions Prim-5 endocardium IHC
mCherry sd12Tg; y7Tg(AB) standard conditions Prim-5 myocardium IHC
sd12Tg; y7Tg + MO5-tal1 + MO6-tal1(AB) standard conditions Prim-5 endocardium IHC
Prim-5 myocardium IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tjp1 IHC sd12Tg; y7Tg(AB) standard conditions Prim-5 endocardium cell-cell junction
IHC Prim-5 myocardium cell-cell junction
IHC sd12Tg; y7Tg + MO5-tal1 + MO6-tal1(AB) standard conditions Prim-5 endocardium cell-cell junction
IHC Prim-5 myocardium cell-cell junction
IHC zn1Tg standard conditions Prim-5 endocardium cell-cell junction
IHC Prim-5 myocardium cell-cell junction
IHC zn1Tg + MO5-tal1 + MO6-tal1 standard conditions Prim-5 endocardium cell-cell junction
IHC Prim-5 myocardium cell-cell junction
Phenotype Details
Fish Conditions Stage Phenotype
sd12Tg; y7Tg + MO5-tal1 + MO6-tal1(AB) standard conditions Prim-5 endocardium cardiac muscle cell differentiation process quality, abnormal
zn1Tg + MO5-tal1 + MO6-tal1 standard conditions Prim-5 endocardium cell-cell junction mislocalised, abnormal
Prim-5 endocardium cell-cell junction organization decreased process quality, abnormal
Prim-5 endocardium endothelial cell obtuse, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 383(2), Schumacher, J.A., Bloomekatz, J., Garavito-Aguilar, Z.V., and Yelon, D., tal1 regulates the formation of intercellular junctions and the maintenance of identity in the endocardium, 214-226, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.