FIGURE

Fig. 3

ID

ZDB-FIG-131031-24

Publication

Özhan et al., 2013 - Lypd6 Enhances Wnt/beta-Catenin Signaling by Promoting Lrp6 Phosphorylation in Raft Plasma Membrane Domains

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Fig. 3

lypd6 Is Necessary for wnt8-Mediated Mesoderm and Neuroectoderm Patterning

(A) Expansion of the dorsal organizer domain (arrows) marked by goosecoid (gsc), not at late blastula (30% epiboly), but at early gastrula (60% epiboly) stage in embryos injected with 10 ng lypd6 MO.

(B) Quantification of the experiment shown in (A). Error bars, SEM.

(C) Changes in the size of the gsc expression domain (arrows) at 60% epiboly in embryos injected with low doses of wnt8.1 and wnt8.2 MOs (1.25 ng each) and lypd6 MO (2 ng), relative to embryos injected with equimolar amounts of control MO.

(D) gsc expression in embryos injected with high doses of wnt8 MOs (5 ng each) and 150 pg lypd6 RNA or equimolar amounts of GFP control RNA.

(E) Quantification of the experiments shown in (C) and (D). Error bars, SEM.

(F) Expansion of the forebrain marker otx2 and reduction of the posterior neural marker hoxb1b at the 100% epiboly stage in embryos injected with 10 ng lypd6 MO (19/23).

Expression Data

Genes:	gsc hoxb1b otx2b
Fish:	WT WT + MO1-lypd6 WT + MO1-wnt8a + MO2-wnt8a WT + MO1-lypd6 + MO1-wnt8a + MO2-wnt8a
Knockdown Reagents:	MO1-lypd6 MO1-wnt8a MO2-wnt8a
Anatomical Terms:	forebrain neural plate neural plate posterior region presumptive dorsal mesoderm shield
Stage Range:	50%-epiboly to Bud

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 26(4), Özhan, G., Sezgin, E., Wehner, D., Pfister, A.S., Kühl, S.J., Kagermeier-Schenk, B., Kühl, M., Schwille, P., and Weidinger, G., Lypd6 Enhances Wnt/beta-Catenin Signaling by Promoting Lrp6 Phosphorylation in Raft Plasma Membrane Domains, 331-345, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell