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Fig. 1

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ZDB-FIG-131031-22

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Özhan et al., 2013 - Lypd6 Enhances Wnt/beta-Catenin Signaling by Promoting Lrp6 Phosphorylation in Raft Plasma Membrane Domains

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Fig. 1

Zebrafish lypd6 Is a Wnt/β-Catenin Target Gene that Codes for a GPI-Anchored Plasma Membrane Protein

(A) lypd6 whole-mount in situ hybridization (WMISH) showing downregulation in transgenic embryos expressing Axin1 or Dkk1 at gastrula and somitogenesis stages (n [85% epiboly]: Axin1 16/17 embryos, Dkk1 19/19; n [10-somites]: Axin1 21/21, Dkk1 18/18). hs, heat shock.

(B) lypd6 expression levels determined by qRT-PCR in hs:Axin1, hs:Dkk1, and hs:Wnt8 transgenic embryos treated as in (A) and shown relative to those in wild-type embryos. Error bars, SEM.

(C) Domain structure of N-terminally GFP-tagged wild-type (spGFP-Lypd6) and C-terminally truncated Lypd6 (spGFP-Lypd6ΔGPI).

(D) Localization of spGFP-Lypd6 and spGFP-Lypd6ΔGPI in enveloping layer (EVL) cells of dome stage zebrafish embryos and in Chinese hamster ovary (CHO) cells.

(E) Release of spGFP-Lypd6 and spGFP-GPI from the plasma membrane of HEK293T cells upon treatment with phosphatidylinositol-specific phospholipase C (PiplC). spGFP-Lypd6ΔGPI and spGFP are detectable in the conditioned media without PiplC treatment.

See also Figure S1.

Expression Data

Gene:	lypd6
Fish:	WT w32Tg w35Tg
Condition:	heat shock
Anatomical Terms:	hypoblast margin somite
Stage Range:	75%-epiboly to 10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 26(4), Özhan, G., Sezgin, E., Wehner, D., Pfister, A.S., Kühl, S.J., Kagermeier-Schenk, B., Kühl, M., Schwille, P., and Weidinger, G., Lypd6 Enhances Wnt/beta-Catenin Signaling by Promoting Lrp6 Phosphorylation in Raft Plasma Membrane Domains, 331-345, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell