FIGURE

Fig. 5

ID

ZDB-FIG-131031-26

Publication

Özhan et al., 2013 - Lypd6 Enhances Wnt/beta-Catenin Signaling by Promoting Lrp6 Phosphorylation in Raft Plasma Membrane Domains

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Lypd6 Interacts with Lrp6 and Partitions into Ordered Membrane Domains in Giant Plasma Membrane Vesicles

(A) spGFP-Lypd6 and spGFP-Lypd6ΔGPI-CD44 TMD coIP with Lrp6-HA, but not with Ldlr-HA, in HEK293T cells. spGFP-Lypd6ΔGPI does not coIP with Lrp6-HA or Ldlr-HA.

(B) spGFP-Lypd6 coIPs with Flag-xFz8 in HEK293T cells, and this binding is strongly enhanced by Wnt3a. Flag-xFz8 also binds to Lrp6-HA, which is enhanced by Wnt3a, but does not bind to Ldlr-HA. Lypd6 and Lrp6 still bind to Fz8 when expressed together.

(C–E) Partitioning of various GFP-tagged Lypd6 constructs in giant plasma membrane vesicles (GPMVs) derived from CHO cells. Fast DiI marks the disordered compartments.

See also Figure S4.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 26(4), Özhan, G., Sezgin, E., Wehner, D., Pfister, A.S., Kühl, S.J., Kagermeier-Schenk, B., Kühl, M., Schwille, P., and Weidinger, G., Lypd6 Enhances Wnt/beta-Catenin Signaling by Promoting Lrp6 Phosphorylation in Raft Plasma Membrane Domains, 331-345, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell