FIGURE

Fig. 8

ID

ZDB-FIG-130910-40

Publication

Yao et al., 2013 - Pbx and Prdm1a transcription factors differentially regulate subsets of the fast skeletal muscle program in zebrafish

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Fig. 8

(A,C,E) RNA in situ expression of (A) myl1 and krox-20, (C) atp2a1 and krox-20, and (E) srl and krox-20 in green, smyhc1 in red, and merged images in control, pbx2/4-MO, prdm1a/, and pbx2/4-MO;prdm1a-/- embryos. krox-20 expression is included as a control for the pbx2/4-MO knockdown (Maves et al., 2007). r3 and r5 indicate krox-20 expression in hindbrain rhombomeres. smyhc1 is downregulated in prdm1a-/- embryos (Elworthy et al., 2008; von Hofsten et al., 2008). (A,C) embryos are at 14 somites, (E) embryos are at 18 somites. All embryos are shown in dorsal view, anterior towards the left. Somites were counted in all embryos shown to confirm consistent staging. (B,D,F) Graphs of real-time RT-PCR (qRT-PCR) expression levels for (B) myl1, (D) atp2a1, and (F) srl. Expression levels were normalized to odc1 expression. Error bars represent standard deviation. Somite stage for qRT-PCR (14s) is shown. Scale bar, 50 μm.

Expression Data

Genes:	atp2a1 egr2b myl1 smyhc1 srl
Fish:	WT prdm1a^m805/m805 WT + MO1-pbx2 + MO2-pbx2 + MO2-pbx4 + MO3-pbx4 prdm1a^m805/m805 + MO1-pbx2 + MO2-pbx2 + MO2-pbx4 + MO3-pbx4
Knockdown Reagents:	MO1-pbx2 MO2-pbx2 MO2-pbx4 MO3-pbx4
Anatomical Terms:	adaxial cell fast muscle cell rhombomere 3 rhombomere 5 slow muscle cell
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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