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ZFIN ID: ZDB-FIG-130907-16
Laux et al., 2013 - Circulating Bmp10 acts through endothelial Alk1 to mediate flow-dependent arterial quiescence. Development (Cambridge, England)   140(16):3403-12 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-25

Fig. 1

Restoration of alk1 expression does not rescue cxcr4a or edn1 expression in the absence of blood flow. (A) Lateral (anterior left), frontal (anterior upwards) and dorsal (anterior left) views of the zebrafish cranial vasculature at 36 hpf. alk1-positive arteries are light gray, alk1-negative arteries are dark gray and veins are black. alk1-positive arteries are closest to the heart. AA1, aortic arch 1; BA, basilar artery; BCA, basal communicating artery; CaDI, caudal division of the internal carotid artery; ICA, internal carotid artery; LDA, lateral dorsal aorta; PCS, posterior communicating segments; PHBC, primordial hindbrain channel; PMBC, primordial midbrain channel. Scale bar: 50 μm. (B) Whole-mount in situ hybridization for cxcr4a, edn1 and cadherin 5 (cdh5, pan-endothelial control) at 36 hpf. cxcr4a and edn1 are upregulated and downregulated, respectively, in alk1 mutants (column 2) and tnnt2a morphants (column 4) compared with controls (column 1), and expression can be normalized to wild-type/control morphant levels by transgene-mediated, flow-independent alk1 expression in alk1 mutants (column 3) but not in tnnt2a morphants (column 5). cdh5 expression is not altered by alk1 or flow conditions. ne50 for all groups. Frontal views, dorsal upwards. Arrows indicate left CaDI. Scale bar: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
acvrl1 WT standard conditions Prim-25 aortic arch 1 ISH
Prim-25 basal communicating artery ISH
Prim-25 caudal division of the internal carotid artery ISH
Prim-25 cranial division of the internal carotid artery ISH
Prim-25 lateral dorsal aorta ISH
cdh5 WT control Prim-25 cranial blood vessel ISH
WT + MO1-tnnt2a standard conditions Prim-25 cranial blood vessel ISH
acvrl1y6/y6 standard conditions Prim-25 cranial blood vessel ISH
acvrl1y6/y6; pt516Tg standard conditions Prim-25 cranial blood vessel ISH
pt516Tg + MO1-tnnt2a standard conditions Prim-25 cranial blood vessel ISH
cxcr4a WT control Prim-25 cranial blood vessel ISH
WT + MO1-tnnt2a standard conditions Prim-25 cranial blood vessel ISH
acvrl1y6/y6 standard conditions Prim-25 cranial blood vessel ISH
acvrl1y6/y6; pt516Tg standard conditions Prim-25 cranial blood vessel ISH
pt516Tg + MO1-tnnt2a standard conditions Prim-25 cranial blood vessel ISH
edn1 WT control Prim-25 cranial blood vessel ISH
WT + MO1-tnnt2a standard conditions Prim-25 Not Detected cranial blood vessel ISH
acvrl1y6/y6 standard conditions Prim-25 Not Detected cranial blood vessel ISH
acvrl1y6/y6; pt516Tg standard conditions Prim-25 cranial blood vessel ISH
pt516Tg + MO1-tnnt2a standard conditions Prim-25 Not Detected cranial blood vessel ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development