FIGURE

Fig. S5

ID

ZDB-FIG-130907-25

Publication

Laux et al., 2013 - Circulating Bmp10 acts through endothelial Alk1 to mediate flow-dependent arterial quiescence

Other Figures

(all 10)

All Figure Page

Back to All Figure Page

Fig. S5

rhBMP10 cannot induce pSmad1/5/9 in the absence of alk1. Intravascular injections of tracer alone or tracer + 2 nl 10 μM rhBMP10 were performed at 28 hpf into wild-type or alk1 mutant embryos. Images show pSmad1/5/9 expression (middle column) in endothelial cells (nuclei marked by fli1a:nEGFP transgene, left column) at 36 hpf. In merge (right column), EGFP-expressing endothelial cell nuclei are green, pSmad1/5/9 immunofluorescence is magenta. Yellow and blue arrows indicate endothelial cells in the CaDI and BCA, respectively. 2D confocal projections of 50 μm frontal sections, dorsal upwards. Scale bar: 50 μm. See Table S1 for fluorescence quantitation.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development