ZFIN ID: ZDB-FIG-130821-8
Anderson et al., 2013 - Hepatocyte Growth Factor Signaling in Intrapancreatic Ductal Cells Drives Pancreatic Morphogenesis. PLoS Genetics   9(7):e1003650 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 4

Cell migration, but not proliferation, is dysregulated in donuts908 mutants.

(A–D) EdU incorporation assay in WT (A,C) and donuts908 mutant (B,D) animals at 56 (A,B) and 75 (C,D) hpf. (E) Quantification of proliferation assay data shows no significant change in acinar cell proliferation at early or late stages of pancreatic tail formation. (F–I) Morphology of exocrine tissues at 84 hpf in WT (F,H) and metMO-injected (G,I) larvae revealed by Tg(ptf1a:GFP) (acinar; F,G) or Tg(nkx2.2a(-3.5 kb):GFP) (duct; H,I) expression. In metMO-injected embryos, acinar and ductal cells fail to migrate caudally, and remain near the principal islet (β). In metMO larvae, ductal cells exhibit a more rounded morphology in the exocrine pancreas (insets) and are also observed in the liver region (asterisk). (J,K) 84 hpf WT (J) and metMO-injected (K) Tg(duct:GFP) larvae stained with Prox1 (blue) and Alcam (red). Pancreatic ductal cells are ectopically localized in the liver (li) along tracts of biliary ducts. (L) Quantification of pancreatic tail defects in small molecule treated larvae; inhibitors of the MAPK pathway (UO126, SB203580) and furin function (CMK) had little effect on pancreatic tail outgrowth, while inhibitors of PI3K (LY294002) and STAT3 (SU6656) function mimicked the type 1 and type 2 donut phenotypes. epd, extrapancreatic duct.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
alcama zn-5 ia3Tg standard conditions Protruding-mouth bile ductule IHC
zn-5 ia3Tg + MO1-met standard conditions Protruding-mouth bile ductule IHC
DsRed jh1Tg; m1018Tg standard conditions Protruding-mouth primary islet IFL
jh1Tg; m1018Tg + MO1-met standard conditions Protruding-mouth primary islet IFL
EGFP jh1Tg standard conditions Long-pec pancreas IFL
Protruding-mouth pancreas IFL
jh1Tg; m1018Tg standard conditions Protruding-mouth pancreas IFL
jh1Tg; m1018Tg + MO1-met standard conditions Protruding-mouth pancreas IFL
mets908/s908; jh1Tg standard conditions Long-pec pancreas IFL
Protruding-mouth pancreas IFL
GFP ia3Tg standard conditions Protruding-mouth extrapancreatic duct IFL
Protruding-mouth intrapancreatic duct IFL
ia3Tg + MO1-met standard conditions Protruding-mouth extrapancreatic duct IFL
Protruding-mouth intrapancreatic duct IFL
Protruding-mouth liver pancreatic ductal cell IFL
prox1a Ab3-prox1 ia3Tg standard conditions Protruding-mouth extrapancreatic duct IHC
Ab3-prox1 Protruding-mouth hepatocyte IHC
Ab3-prox1 ia3Tg + MO1-met standard conditions Protruding-mouth extrapancreatic duct IHC
Ab3-prox1 Protruding-mouth hepatocyte IHC
Ab3-prox1 Protruding-mouth liver pancreatic ductal cell IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ia3Tg + MO1-met standard conditions Protruding-mouth exocrine pancreas development disrupted, abnormal
Protruding-mouth pancreas spheroid, abnormal
Protruding-mouth pancreatic duct cell migration disrupted, abnormal
Protruding-mouth pancreatic duct morphology, abnormal
Protruding-mouth pancreatic ductal cell located in hepatic duct, abnormal
Protruding-mouth pectoral fin musculature decreased tonicity, abnormal
jh1Tg; m1018Tg + MO1-met standard conditions Protruding-mouth pancreas spheroid, abnormal
Protruding-mouth pancreatic duct cell migration disrupted, abnormal
Protruding-mouth pancreatic duct morphology, abnormal
Protruding-mouth pancreatic ductal cell located in hepatic duct, abnormal
mets908/s908; jh1Tg standard conditions Long-pec exocrine pancreas cell migration disrupted, abnormal
Long-pec exocrine pancreas cell population proliferation process quality, normal
Long-pec exocrine pancreas development disrupted, abnormal
Long-pec pancreas spheroid, abnormal
Protruding-mouth exocrine pancreas cell migration disrupted, abnormal
Protruding-mouth exocrine pancreas cell population proliferation process quality, normal
Protruding-mouth exocrine pancreas development disrupted, abnormal
Protruding-mouth pancreas spheroid, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.