Fig. 1

cmp^s819 controls endodermal differentiation and global transcription. (A) Schematic of endodermal organs. (B,C) Confocal projections of endodermal Tg(XlEef1a1:GFP)^s854, hepatic and pancreatic Prox1 and 2F11 in the extrahepatopancreatic ducts (EHPD) show liver and pancreas hypoplasia in cmp^s819 mutants. (D) Prox1-positive hepatoblasts are significantly reduced in cmp^s819 embryos at 48 hpf; data show mean±s.e.m., ^***P=1.2^-5. (E-H) At 48 hpf, cp is expressed in cmp^s819 livers (E,F), but undetectable at 96 hpf (G,H). (I,J) gc expression is absent in cmp^s819 embryos at 60 hpf. (K-N) foxa1 is expressed throughout the cmp^s819 endoderm at 40 hpf (K,L) and is absent at 72 hpf, whereas residual foxa1 is detectable in neural tissues (M,N). (O,P) Tg(XIEef1a1:GFP)^s854 labels the digestive system in sibling and cmp^s819 embryos at 72 hpf. (Q-R3) EU incorporation reveals reduced RNA transcription in the digestive system of cmp^s819 mutants at 48 hpf. Using the same confocal settings, magnifications of representative sections show fewer EU-positive foci (arrows) in cmp^s819 livers compared with controls. A-C,O-R3 are ventral views; E-N are dorsal views; all show anterior to the top. Arrowheads indicate liver.