FIGURE

Fig. 2

ID

ZDB-FIG-130802-13

Publication

Koltowska et al., 2013 - Ssrp1a controls organogenesis by promoting cell cycle progression and RNA synthesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

ssrp1a is disrupted in cmp mutants. (A) Genetic map of the cmp-containing region. s819- and u428-lesions in ssrp1a are shown at the nucleotide and protein level. (B-G) MO-ssrp1a-injected embryos (F,G) phenocopy cmp^s819 defects (D,E). Lateral views show smaller heads and eyes in mutant (D) and MO-ssrp1a embryos (F) compared with controls (B). Confocal projections of Tg(XIEef1a1:GFP)^s854 highlight liver (arrowheads) and pancreas hypoplasia in cmp^s819 (E), and MO-ssrp1a injected embryos (G) compared with controls (C); ventral views, anterior to the top. (H-L) ssrp1a expression in wild-type embryos between 2.5 and 72 hpf. Dorsal views, anterior to the top. After 24 hpf, ssrp1a is enriched in the liver (arrowheads), eyes and fins. (M) qPCR analyses show dynamic ssrp1a and ssrp1b expression levels between 2.5 and 120 hpf. Error bars represent s.d.

Expression Data

Genes:	EGFP ssrp1a ssrp1b
Fish:	WT s854Tg s854Tg + MO2-ssrp1a ssrp1a^s819/s819; s854Tg
Knockdown Reagent:	MO2-ssrp1a
Anatomical Terms:	eye gut liver pectoral fin unspecified whole organism
Stage Range:	256-cell to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s854Tg + MO2-ssrp1a ssrp1a^s819/s819; s854Tg
Knockdown Reagent:	MO2-ssrp1a
Observed In:	DNA-templated transcription eye head liver
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development