FIGURE

Fig. 1

ID

ZDB-FIG-130730-1

Publication

Wilfinger et al., 2013 - Cell type and tissue specific function of islet genes in zebrafish pancreas development

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

Reduced expression of endocrine hormones in isl1 mutants. (A–F) Confocal image projections of the pancreatic islet in control (A–C) and isl1^-/- embryos (D–F) that were co-immunostained for Ins (green) and Sst (red) at 28 hpf (A, D), 48 hpf (B, E) and 76 hpf (C, F). (G–L) Immunostainings for Gcg (purple) in control (G–I) and in isl1^-/- embryos (J–L) at 48 hpf (G, J), 76 hpf (H, K) and 96 hpf (I, L). All embryos are shown from ventral with the anterior to the left. (M) Quantitative analysis of hormone expressing cells in control and isl1^-/- embryos. Bars show mean+SEM. (N) Relative expression levels of ins, gcga and sst2 mRNA in isl1^-/- mutant fish in relation to control littermates as revealed by qPCR analyses of whole embryo RNA preparations, normalized to EF1α.

Expression Data

Antibodies:	Ab1-ins Ab2-gcg Ab3-sst
Fish:	WT isl1a^sa29/sa29
Anatomical Terms:	endocrine pancreas glucagon secreting cell endocrine pancreas insulin secreting cell endocrine pancreas somatostatin secreting cell posterior pancreatic bud insulin secreting cell posterior pancreatic bud somatostatin secreting cell
Stage Range:	Prim-5 to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	isl1a^sa29/sa29
Observed In:	endocrine pancreas glucagon secreting cell insulin secreting cell posterior pancreatic bud somatostatin secreting cell
Stage Range:	Prim-5 to Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 378(1), Wilfinger, A., Arkhipova, V., and Meyer, D., Cell type and tissue specific function of islet genes in zebrafish pancreas development, 25-37, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.