ZFIN ID: ZDB-FIG-130625-25
Nord et al., 2013 - Six1 regulates proliferation of Pax7-positive muscle progenitors in zebrafish. Journal of Cell Science   126(Pt 8):1868-80 Full text @ J. Cell Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Fig. 2 six1 morphants have a reduced fast muscle area. (A) Ventral and lateral views of head (left two columns) and lateral view and cross-section of the trunk (right two columns) of 3-dpf tg(mylz2:GFP)i135 embryos uninjected and injected with six1a and six1b morpholinos alone or in combination. Cross-sections are stained with F310. (B) F310+ cross-section area of 24-hpf and 3-dpf embryos; the area of uninjected control embryos is set as 100%. Compared with control embryos at 24hpf (n = 5), six1a morphants have an area of 94.0% (n = 5), six1b morphants 88.4% (n = 5) and six1a and six1b double morphants 92.8% (n = 5). In 3-dpf embryos compared with control embryos, (n = 5) six1a morphants have an area of 84.9% (n = 5), six1b morphants 55.0% (n = 5) and six1a/six1b double morphants 37.2% (n = 6). Compared with uninjected control embryos (n = 4) set as 100%, six1a/p53 morphants have an F310+ cross-section area of 79.7% (n = 4), six1b/p53 morphant embryos 62.1% (n = 5) and six1a/six1b/p53 triple morphants have an area of 55.6% (n = 4). (C) Average number of DAPI-stained nuclei per F310+ (fast muscle specific) cross-section area of 3-dpf embryos. Uninjected control embryos have an average of 151.3 DAPI-stained nuclei per F310+ cross-section area (n = 5), six1a morphants have 156.0 (n = 5), six1b morphants 135.2 (n = 6) and six1a/six1b double morphants 119.5 (n = 7). 3-dpf six1a/p53 morphants have an average number of 156 DAPI-stained nuclei per F310+ cross-section area (n = 4), six1b/p53 morphants 152.2 (n = 5), six1a/six1b/p53 triple morphants 119.3 (n = 4) and control embryos have 159.5 (n = 4). (D) Number of slow fibres per somite in 24-hpf and 3-dpf embryos. fb, fin bud; hh, hyohyoideus; ih, interhyoideus; ima, intermandibular anterior; imp, intermandibular posterior; om, ocular muscles; sh, sternohyoideus; som, somites. Cross-sections were made at somite number 10 for 24-hpf embryos, and at somite number 8 for 3-dpf embryos. *P<0.05, **P<0.01, ***P<0.001. Scale bars: 50μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP i135Tg standard conditions Protruding-mouth extraocular musculature IHC
Protruding-mouth head muscle IHC
Protruding-mouth hyohyoideus IHC
Protruding-mouth interhyoideus IHC
Protruding-mouth paired fin muscle IHC
Protruding-mouth sternohyoid IHC
Protruding-mouth trunk muscle IHC
Protruding-mouth ventral intermandibularis anterior IHC
Protruding-mouth ventral intermandibularis posterior IHC
i135Tg + MO1-six1a standard conditions Protruding-mouth hyohyoideus IHC
Protruding-mouth interhyoideus IHC
Protruding-mouth paired fin muscle IHC
Protruding-mouth sternohyoid IHC
Protruding-mouth trunk muscle IHC
Protruding-mouth ventral intermandibularis posterior IHC
i135Tg + MO1-six1a + MO4-six1b standard conditions Protruding-mouth trunk muscle IHC
i135Tg + MO4-six1b standard conditions Protruding-mouth trunk muscle IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-F310 IHC i135Tg standard conditions Protruding-mouth fast muscle cell
IHC Protruding-mouth trunk muscle
IHC i135Tg + MO1-six1a standard conditions Protruding-mouth fast muscle cell
IHC Protruding-mouth trunk muscle
IHC i135Tg + MO1-six1a + MO4-six1b standard conditions Protruding-mouth fast muscle cell
IHC Protruding-mouth trunk muscle
IHC i135Tg + MO4-six1b standard conditions Protruding-mouth fast muscle cell
IHC Protruding-mouth trunk muscle
Phenotype Details
Fish Conditions Stage Phenotype
i135Tg + MO1-six1a standard conditions Prim-5 fast muscle cell muscle cell development decreased process quality, abnormal
Prim-5 slow muscle cell normal amount, normal
Protruding-mouth cephalic musculature skeletal muscle tissue development decreased process quality, abnormal
Protruding-mouth fast muscle cell muscle cell development decreased process quality, abnormal
Protruding-mouth slow muscle cell normal amount, normal
i135Tg + MO1-six1a + MO4-six1b standard conditions Prim-5 fast muscle cell muscle cell development decreased process quality, abnormal
Prim-5 slow muscle cell normal amount, normal
Protruding-mouth cephalic musculature skeletal muscle tissue development decreased process quality, abnormal
Protruding-mouth fast muscle cell decreased amount, abnormal
Protruding-mouth fast muscle cell muscle cell development decreased process quality, abnormal
Protruding-mouth slow muscle cell normal amount, normal
i135Tg + MO1-six1a + MO4-six1b + MO4-tp53 standard conditions Prim-5 fast muscle cell muscle cell development decreased process quality, abnormal
Protruding-mouth fast muscle cell decreased amount, abnormal
Protruding-mouth fast muscle cell muscle cell development decreased process quality, abnormal
i135Tg + MO1-six1a + MO4-tp53 standard conditions Prim-5 fast muscle cell muscle cell development decreased process quality, abnormal
Protruding-mouth fast muscle cell muscle cell development decreased process quality, abnormal
i135Tg + MO4-six1b standard conditions Prim-5 fast muscle cell muscle cell development decreased process quality, abnormal
Prim-5 slow muscle cell normal amount, normal
Protruding-mouth cephalic musculature skeletal muscle tissue development decreased process quality, abnormal
Protruding-mouth fast muscle cell muscle cell development decreased process quality, abnormal
Protruding-mouth slow muscle cell normal amount, normal
i135Tg + MO4-six1b + MO4-tp53 standard conditions Prim-5 fast muscle cell muscle cell development decreased process quality, abnormal
Protruding-mouth fast muscle cell muscle cell development decreased process quality, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.