FIGURE

Fig. 4

ID

ZDB-FIG-130625-27

Publication

Nord et al., 2013 - Six1 regulates proliferation of Pax7-positive muscle progenitors in zebrafish

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Fig. 4

six1b expression in Pax7⁺ cells is independent of MyoD and Myf5. (A) Cross-section of uninjected control embryos and myoD/myf5 morphant embryos at 24hpf showing mRNA expression of six1b (red) together with slow muscle marker F59 (green) or fast muscle marker F310 (green) and of Pax7 alone. six1b expression in the Pax7⁺ domain remains in MyoD/Myf5 double morphants. (B) Cross-section of control and myoD/myf5 morphant six1b:GFP embryos at 24hpf showing expression of Pax7 (red) together with six1b:GFP (green). six1b:GFP and Pax7 expression domains coincide in the myoD/myf5 morphants. Cross-sections were made at somite number 10. Scale bars: 50 μm.

Expression Data

Genes:	GFP six1b
Antibodies:	Ab-F59 Ab-F310 Ab-Pax7
Fish:	WT umu1Tg WT + MO1-myod1 + MO3-myf5 umu1Tg + MO1-myod1 + MO3-myf5
Knockdown Reagents:	MO1-myod1 MO3-myf5
Anatomical Terms:	fast muscle cell myoblast slow muscle cell somite
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-myod1 + MO3-myf5
Knockdown Reagents:	MO1-myod1 MO3-myf5
Observed In:	fast muscle cell muscle cell development slow muscle cell muscle cell development
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.