ZFIN ID: ZDB-FIG-130409-10
Zhang et al., 2013 - Control of hematopoietic stem cell emergence by antagonistic functions of ribosomal protein paralogs. Developmental Cell   24(4):411-425 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Protruding-mouth to Day 5

Fig. 3

Distinct, Lineage-Restricted Defects in Hematopoiesis in rpl22 and rpl22l1 Morphants (A and B) WISH analysis of thymocyte development with the indicated probes in rpl22 (A) and rpl22l1 morphants (B) at 5 dpf. Thymus, red dashed rectangles. (C–E) Evaluation of thymus colonization and HSC emergence in rpl22l1 and rpl22 morphants. Tg(cd41:EGFP) embryos were injected with Rpl22l1 MO, Rpl22 MO, or MM control, after which seeding of the thymus and HSC emergence was assessed at 3.5 dpf by tracking the presence of GFP+ cells in the thymus (red circles) or VDA (between the red and white dashed lines). Emerging HSC are indicated by arrows. The area between the dashed white and yellow lines is the pronephric duct. The number of CD41-EGFPlow HSCs was quantified in six representative embryos per group and presented as mean ± SD. *p < 0.001. Images depict phenotypes representative of at least three separate experiments, with numbers referring to the fraction of morphants with the depicted phenotypes. See also Figure 3.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP la2Tg control Protruding-mouth hematopoietic stem cell ISH
Protruding-mouth pronephric duct cell ISH
Protruding-mouth thymus ISH
Protruding-mouth ventral wall of dorsal aorta ISH
la2Tg + MO1-rpl22 standard conditions Protruding-mouth pronephric duct cell ISH
Protruding-mouth thymus ISH
Protruding-mouth ventral wall of dorsal aorta ISH
la2Tg + MO1-rpl22l1 standard conditions Protruding-mouth pronephric duct cell ISH
Protruding-mouth Not Detected thymus ISH
Protruding-mouth Not Detected ventral wall of dorsal aorta ISH
ikzf1 WT control Day 5 common lymphoid progenitor ISH
WT + MO1-rpl22 standard conditions Day 5 common lymphoid progenitor ISH
WT + MO1-rpl22l1 standard conditions Day 5 Not Detected common lymphoid progenitor ISH
lck WT control Day 5 pro-T cell ISH
Day 5 T cell ISH
WT + MO1-rpl22 standard conditions Day 5 Not Detected pro-T cell ISH
Day 5 Not Detected T cell ISH
WT + MO1-rpl22l1 standard conditions Day 5 Not Detected pro-T cell ISH
Day 5 Not Detected T cell ISH
rag1 WT control Day 5 common lymphoid progenitor ISH
WT + MO1-rpl22 standard conditions Day 5 common lymphoid progenitor ISH
WT + MO1-rpl22l1 standard conditions Day 5 Not Detected common lymphoid progenitor ISH
trbc2 WT control Day 5 alpha-beta T cell ISH
Day 5 T cell ISH
WT + MO1-rpl22 standard conditions Day 5 Not Detected alpha-beta T cell ISH
Day 5 Not Detected T cell ISH
WT + MO1-rpl22l1 standard conditions Day 5 Not Detected alpha-beta T cell ISH
Day 5 Not Detected T cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-rpl22 standard conditions Day 5 alpha-beta T cell aplastic/hypoplastic, abnormal
Day 5 alpha-beta T cell differentiation arrested, abnormal
Day 5 T cell aplastic/hypoplastic, abnormal
Day 5 T cell differentiation arrested, abnormal
WT + MO1-rpl22l1 standard conditions Day 5 alpha-beta T cell aplastic/hypoplastic, abnormal
Day 5 alpha-beta T cell differentiation arrested, abnormal
Day 5 gamma-delta T cell aplastic/hypoplastic, abnormal
Day 5 gamma-delta T cell differentiation arrested, abnormal
Day 5 T cell aplastic/hypoplastic, abnormal
Day 5 T cell differentiation arrested, abnormal
la2Tg + MO1-rpl22 standard conditions Protruding-mouth ventral wall of dorsal aorta hematopoietic stem cell differentiation process quality, normal
la2Tg + MO1-rpl22l1 standard conditions Protruding-mouth ventral wall of dorsal aorta hematopoietic stem cell differentiation decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 24(4), Zhang, Y., Duc, A.C., Rao, S., Sun, X.L., Bilbee, A.N., Rhodes, M., Li, Q., Kappes, D.J., Rhodes, J., and Wiest, D.L., Control of hematopoietic stem cell emergence by antagonistic functions of ribosomal protein paralogs, 411-425, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell