FIGURE

Fig. 1

ID
ZDB-FIG-130208-8
Publication
Langworthy et al., 2012 - Schwann cell myelination requires Dynein function
Other Figures
All Figure Page
Back to All Figure Page
Fig. 1

dync1h1 -deficient larvae express mbp RNA and protein in the central nervous system (CNS) but not the peripheral nervous system (PNS). (A, B) mbp RNA expression in the ventral spinal cord (white arrowhead), along motor nerves (mn, asterisks) and at the posterior lateral line nerve (pLLn, black arrowhead) of wild-type larvae. (C) Immunohistochemistry reveals Mbp (green) associated with motor nerves marked by Acetylated Tubulin staining (red, brackets). Arrows indicate prominent Mbp in the ventral spinal cord. The inset shows Mbp expression, alone, of the portion of the motor nerve, indicated by the dashed box. (D, E) mbp RNA expression in the spinal cord but not at the motor nerves or the pLLn of dync1h1 mutant larvae. (F) Mbp is absent from motor nerves and reduced in the ventral spinal cord of a mutant larva. The inset shows Mbp labeling, alone, of the portion of the motor nerve indicated by the dashed box. (G) pLLn of a wild-type larvae injected with dync1h1 antisense MO lacks mbp RNA expression. Panels A, B, D, E and G show whole 4.25 days post fertilization (dpf) larvae at the level of the mid-trunk with anterior to the left and dorsal to the top. Panels C and F show transverse sections through the level of the trunk with dorsal up. Scale bars equal 20 μm (A, B, D, E, G), 40 μm (C, F) and 50 μm for the insets.

Expression Data
Gene:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Day 4 to Day 6

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Neural Dev.