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ZFIN ID: ZDB-FIG-130125-2
Liu et al., 2012 - Prdm14 acts upstream of islet2 transcription to regulate axon growth of primary motoneurons in zebrafish. Development (Cambridge, England)   139(24):4591-4600 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage Range: 10-13 somites to 20-25 somites

Fig. S9

Prdm14 is a nuclear protein and prdm14 is expressed before islet2 in zebrafish embryos. (A) Zebrafish Prdm14 is localized to the nucleus, similar to human PRDM14. The localization is ZF domain dependent. (B) The localization of different zebrafish Prdm14 isoforms is summarized. N, nucleus localized; C, cytoplasm localized. (C) prdm14 is expressed earlier than islet2 in CaP (arrows) at the 10-somite stage. Prdm14 downregulation causes decreased islet2 expression at the 20-somite stage. (D) Diagram illustrating the results in C. Scale bars: 10 µm in A; 200 µm in C.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
isl2a WT standard conditions 10-13 somites Not Detected CaP motoneuron ISH
20-25 somites CaP motoneuron ISH
prdm14gd1Gt/gd1Gt standard conditions 10-13 somites Not Detected CaP motoneuron ISH
20-25 somites CaP motoneuron ISH
prdm14 WT standard conditions 10-13 somites CaP motoneuron ISH
20-25 somites CaP motoneuron ISH
prdm14gd1Gt/gd1Gt standard conditions 10-13 somites CaP motoneuron ISH
20-25 somites CaP motoneuron ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development