Reduction of Prdm14 expression causes embryonic movement defects. (A) Frequencies of spontaneous tail coiling in slg mutant and splmo morphant embryos are significantly reduced compared with wild-type and heterozygous embryos. The normal tail coiling is restored in revertant embryos. Ten embryos were examined in each group. Error bars indicate s.e.m. (B) The percentage of full tail coil in slg mutant and splmo morphant embryos after touch stimulus is significantly decreased compared with wild-type and heterozygous embryos. The normal movements were largely restored in revertant embryos. A total of 30-45 touch responses were monitored in each embryo and the percentage of full tail coil calculated. In each group, at least three embryos were recorded. Error bars indicate s.e.m. of triplicate experiments. ***P<0.001; ns, not significant; compared with wild type (two-tailed unpaired t-test). (C) Frame shots of typical twist movement in the touch response. See supplementary material Movies 1-5. Scale bars: 200 μm.