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ZFIN ID: ZDB-FIG-130117-20
Liu et al., 2012 - Prdm14 acts upstream of islet2 transcription to regulate axon growth of primary motoneurons in zebrafish. Development (Cambridge, England)   139(24):4591-4600 Full text @ Development
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. 4 CaP axons are shortened in slg mutant and splmo morphant zebrafish embryos. (A) CaP axon projection in 26-hpf wild type. Four regions are defined to describe CaP axon outgrowth: HMS, horizontal myoseptum; VNC, myotome adjacent to the ventral edge of the notochord; PVM and DVM, proximal and distal portion of the ventral myotome. (B-D2) slg mutant and splmo morphant embryos show greatly shortened CaP axons at 26 hpf. The boxed regions are magnified in B2-D2. Dashed line indicates horizontal septum. (E) znp1 immunostaining shows that the shortened axons are CaP in slg mutant and splmo morphant embryos. (F) Summary of CaP axon outgrowth (hemisegments 8-12) in slg heterozygous, slg homozygous, splmo morphant and slg revertant embryos. For each group, a total of 25 axons from five embryos were scored as HMS, VNC, PVM or DVM. Error bars indicate s.e.m. of triplicate experiments. Lateral views, dorsal to the top and anterior to the left. Arrows indicate shortened CaP axons; arrowheads indicate abnormal branched axons. Scale bars: 20 μm in B-D′; 50 μm in E.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-prdm14 standard conditions Prim-5 CaP motoneuron axon decreased length, abnormal
Prim-5 collateral sprouting process quality, abnormal
prdm14gd1Gt/gd1Gt standard conditions Prim-5 CaP motoneuron axon decreased length, abnormal
Prim-5 collateral sprouting process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development