ZFIN ID: ZDB-FIG-130109-12
Ichimura et al., 2012 - Structural disorganization of pronephric glomerulus in zebrafish mpp5a/nagie oko mutant. Developmental dynamics : an official publication of the American Association of Anatomists   241(12):1922-1932 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 7 A tnnt2a-ATG morphant displays glomerulus disorganization similar to that found in the mpp5am520 mutants. Pronephric glomerular structure and nephrin mRNA expression are shown by hematoxylin-eosin stained section (A-F) and in situ hybridization with eosin-stained (G-J), respectively. In wild-type (A, C, E, G, I), a pair of glomerular primordia has already merged to form a single glomerulus (arrowheads) beneath the notochord at 2 dpf (A, G), 3 dpf (C, I), and 4 dpf (E). In tnnt2a-ATG morphants (B, D, F, H, J), a pair of glomerular primordia has retained their epithelial vesicular structure and remains unmerged at the midline in 2 dpf (arrows in B, H). Glomerular primordia have still not merged, and an extremely dilated dorsal aorta is interposed between glomerular primordia at 3 dpf (D) and 4 dpf (F). Bowman′s space is apparent in most cases of tnnt2a-ATG morphants (D, F). nephrin-expressing regions are moustache-like in shape and expanded in the direction of the pronephric tubule (arrows in H, I), as is the case in the mpp5a mutants. Scale bar = 10 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
nphs1 WT control Long-pec pronephric podocyte ISH
Protruding-mouth pronephric podocyte ISH
WT + MO1-tnnt2a standard conditions Long-pec pronephric podocyte ISH
Protruding-mouth pronephric podocyte ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-tnnt2a standard conditions Long-pec dorsal aorta dilated, abnormal
Long-pec pronephric glomerulus disorganized, abnormal
Long-pec pronephric glomerulus hypoplastic, abnormal
Long-pec pronephric glomerulus unfused from pronephric glomerulus, abnormal
Long-pec pronephric podocyte nphs1 expression spatial pattern, abnormal
Protruding-mouth dorsal aorta dilated, abnormal
Protruding-mouth pronephric glomerulus disorganized, abnormal
Protruding-mouth pronephric glomerulus hypoplastic, abnormal
Protruding-mouth pronephric glomerulus unfused from pronephric glomerulus, abnormal
Protruding-mouth pronephric podocyte nphs1 expression spatial pattern, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.