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ZIRC
ZFIN ID: ZDB-FIG-121214-1
Fior et al., 2012 - The differentiation and movement of presomitic mesoderm progenitor cells are controlled by Mesogenin 1. Development (Cambridge, England)   139(24):4656-4665 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: 14-19 somites
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: 14-19 somites

Fig. 1 Msgn1 and Spt are essential for tail somite formation. (A-D,E-H) Live zebrafish embryos typical of their genotypic classes. (A′-D′′ ′,E′-H′′ ′ ′) In situ hybridisation for ntl and cb1045 (xirp2a), myoD (myod1), tbx24 and mespaa expression in uninjected wild-type (wt) siblings and in the genotypes indicated. spt-/- mutants were derived from a heterozygous spt+/- cross. msgn1-/- mutants, spt+/-;msgn1-/- mutants and spt-/-;msgn1-/- double mutants were derived from a double heterozygous msgn1+/-;spt+/- cross. Indicated is the number of embryos (n) observed with the phenotype shown in each panel, and when embryos were derived from mutant crosses the obtained n corresponded to the expected frequencies for each genotype. Asterisk indicates that wt and msgn1 mutants have an undistinguishable phenotype at the 14-somite stage.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
mespaa WT standard conditions 14-19 somites segmental plate anterior-most region ISH
msgn1fh273/fh273 standard conditions 14-19 somites segmental plate anterior-most region ISH
msgn1fh273/fh273; tbx16b104/+ standard conditions 14-19 somites segmental plate anterior-most region ISH
msgn1fh273/fh273; tbx16b104/b104 standard conditions 14-19 somites Not Detected segmental plate ISH
myod1 WT standard conditions 14-19 somites somite ISH
WT + MO1-msgn1 standard conditions 14-19 somites somite ISH
msgn1fh273/fh273 standard conditions 14-19 somites somite ISH
msgn1fh273/fh273; tbx16b104/+ standard conditions 14-19 somites somite ISH
msgn1fh273/fh273; tbx16b104/b104 standard conditions 14-19 somites Not Detected somite ISH
tbx16b104/b104 standard conditions 14-19 somites somite ISH
tbx16b104/b104 + MO1-msgn1 standard conditions 14-19 somites Not Detected somite ISH
tbx6 WT standard conditions 14-19 somites segmental plate anterior region ISH
WT + MO1-msgn1 standard conditions 14-19 somites segmental plate anterior region ISH
msgn1fh273/fh273 standard conditions 14-19 somites segmental plate anterior region ISH
msgn1fh273/fh273; tbx16b104/+ standard conditions 14-19 somites segmental plate anterior region ISH
msgn1fh273/fh273; tbx16b104/b104 standard conditions 14-19 somites Not Detected segmental plate ISH
tbx16b104/b104 standard conditions 14-19 somites segmental plate anterior region ISH
tbx16b104/b104 + MO1-msgn1 standard conditions 14-19 somites segmental plate anterior region ISH
tbxta WT standard conditions 14-19 somites notochord ISH
14-19 somites tail bud ISH
WT + MO1-msgn1 standard conditions 14-19 somites notochord ISH
14-19 somites tail bud ISH
msgn1fh273/fh273 standard conditions 14-19 somites notochord ISH
14-19 somites tail bud ISH
msgn1fh273/fh273; tbx16b104/+ standard conditions 14-19 somites notochord ISH
14-19 somites tail bud ISH
msgn1fh273/fh273; tbx16b104/b104 standard conditions 14-19 somites notochord ISH
14-19 somites tail bud ISH
tbx16b104/b104 standard conditions 14-19 somites notochord ISH
14-19 somites tail bud ISH
tbx16b104/b104 + MO1-msgn1 standard conditions 14-19 somites notochord ISH
14-19 somites tail bud ISH
xirp2a WT standard conditions 14-19 somites somite border ISH
WT + MO1-msgn1 standard conditions 14-19 somites somite border ISH
msgn1fh273/fh273 standard conditions 14-19 somites somite border ISH
msgn1fh273/fh273; tbx16b104/+ standard conditions 14-19 somites Not Detected somite ISH
msgn1fh273/fh273; tbx16b104/b104 standard conditions 14-19 somites Not Detected somite ISH
tbx16b104/b104 standard conditions 14-19 somites somite border ISH
tbx16b104/b104 + MO1-msgn1 standard conditions 14-19 somites Not Detected somite ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-msgn1 standard conditions 14-19 somites tail bud cellular quality, abnormal
msgn1fh273/fh273 standard conditions 14-19 somites tail bud cellular quality, abnormal
msgn1fh273/fh273; tbx16b104/+ standard conditions 14-19 somites somitogenesis decreased process quality, abnormal
msgn1fh273/fh273; tbx16b104/+ standard conditions 14-19 somites tail bud increased size, abnormal
msgn1fh273/fh273; tbx16b104/b104 standard conditions 14-19 somites post-vent region lacks all parts of type somite, abnormal
14-19 somites somitogenesis arrested, abnormal
14-19 somites tail bud increased size, abnormal
tbx16b104/b104 standard conditions 14-19 somites tail bud increased size, abnormal
tbx16b104/b104 + MO1-msgn1 standard conditions 14-19 somites post-vent region lacks all parts of type somite, abnormal
14-19 somites somitogenesis arrested, abnormal
14-19 somites tail bud increased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development