Fig. 1

Fig. 1 Loss of function of dpysl2 and dpysl3 inhibited the proper positioning of Rohon-Beard neurons in the dorsal part of the spinal cord. (A–H) Gene expression pattern of dpysl2 (A–D) and dpsyl3 (E–H) at 12 hpf (A, B, E, F) and at 28 hpf (C, D, G, H). Lateral view, anterior left (A, C, E, G) and dorsal view, anterior top (B, D, F, H). Arrowheads in C, D, G, H indicate RB neurons. (I–L) The position of RB neurons in the dorsal spinal cord in the wild-type (I) and the morphants (J–L) at 28 hpf. Dorsal view, anterior top. In the morphants, the position of RB neurons shifted medially compare to the wild-type embryos. (M, N) Quantitative data for the percentage of RB neurons on the midline (M), and total nomber of RB neurons within 3 segments (N). Data are mean ± SEM; ^NNP<0.01, ^NNNP<0.001, different from the wild-type embryos using 2-tailed paired Student′s t-test.