ZFIN ID: ZDB-FIG-120815-57
Tsai et al., 2012 - The paracrine effect of exogenous growth hormone alleviates dysmorphogenesis caused by tbx5 deficiency in zebrafish (Danio rerio) embryos. Journal of Biomedical Science   19(1):63 Full text @ J. Biomed. Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 6 Expressions of cardiomyogenesis-related genes in GH-treatedtbx5mutants. Expressions of (A)amhc, (B)vmhc,and (C)cmlc2in MO group zebrafish embryos were depressed throughout the developmental stages compared to WT zebrafish embryos. Expression of these thre cardiac myogenesis genes showed significant restorative effects in MOGH group embryos. On the other hand, expressions of cardiac myogenesis genes were not significantly affected in WTGH group embryos. The expression of amhc was restricted to the atrium in WT embryos (D), and its expression was depressed in MO group embryos (G). (J) MOGH group zebrafish embryos showed an ameliorating effect on restoration by the expression of amhc. The expression of vmhc was restricted to the ventricle in WT embryos (E) but showed minor expression in MO group embryos (H). (K) However, the expression of vmhc was significantly induced in the MOGH group. The expression pattern of cmlc2 was similar to that of vmhc, in that expression was low in tbx5-deficient embryos (I) but was restored in GH-treated embryos (L). Schematics indicate the location of the expressions of amhc (L), vmhc (M), and cmlc2 (N). Data are presented as the mean ± S.D. *p < 0.05 vs. WT. WT, wild-type embryos; MO, tbx5 knockdown; MIS, mismatched tbx5-MO-treated embryos; WTGH, WT embryos treated with growth hormone (GH); MOGH, tbx5-MO- and GH-treated embryos; a, atrium; v, ventricle.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myh6 WT control Long-pec atrium ISH
WT + MO1-tbx5a standard conditions Long-pec atrium ISH
WT + MO1-tbx5a chemical treatment by injection: growth hormone Long-pec atrium ISH
myh7 WT control Long-pec cardiac ventricle ISH
WT + MO1-tbx5a standard conditions Long-pec cardiac ventricle ISH
WT + MO1-tbx5a chemical treatment by injection: growth hormone Long-pec cardiac ventricle ISH
myl7 WT control Long-pec cardiac ventricle ISH
WT + MO1-tbx5a standard conditions Long-pec cardiac ventricle ISH
WT + MO1-tbx5a chemical treatment by injection: growth hormone Long-pec cardiac ventricle ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-tbx5a standard conditions Long-pec atrium myh6 expression decreased amount, abnormal
Long-pec cardiac ventricle myh7 expression decreased amount, abnormal
Long-pec cardiac ventricle myl7 expression decreased amount, abnormal
WT + MO1-tbx5a chemical treatment by injection: growth hormone Long-pec atrium myh6 expression amount, ameliorated
Long-pec cardiac ventricle myh7 expression amount, ameliorated
Long-pec cardiac ventricle myl7 expression amount, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Journal of Biomedical Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Biomed. Sci.