ZFIN ID: ZDB-FIG-120815-55
Tsai et al., 2012 - The paracrine effect of exogenous growth hormone alleviates dysmorphogenesis caused by tbx5 deficiency in zebrafish (Danio rerio) embryos. Journal of Biomedical Science   19(1):63 Full text @ J. Biomed. Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Term:
Stage: Prim-15
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Prim-15

Fig. 4

Expression of apoptosis-related genes and proteins in tbx5 knockdown and growth hormone (GH)-treated zebrafish embryos at 30 h post-fertilization (hpf). Using a semiquantitative RT-PCR, the apoptotic genes, bcl2 (A) and bad (B), were significantly induced in MO group embryos and showed no significant differences among the WT, WTGH, and MOGH groups. (n = 3, 50 embryos/stage; relative expression = gene expression/β-actin expression). (C-R) Zebrafish embryos were stained by apoptosis-related antibodies, Bad and Bcl2 (red), and counterstained with DAPI (blue) for nucleus observation. In sagittal sections of the heart, Bad and Bcl2 were similarly expressed and significantly induced in tbx5-deficienct embryos (E, I), and showed no significant differences among the WT (C, G), WTGH (D, H), and MOGH (F, J) groups. Transverse sections showed that expression patterns of Bad and Bcl2 in pectoral fins were significantly induced in tbx5-deficient embryos (M, Q) and showed insignificant differences among the WT (K, O), WTGH (L, P), and MOGH (N, R) groups. (C-R) Embryo anteriors are to the left. WT, wild-type embryos; MO, tbx5 knockdown; MIS, mismatch tbx5-MO-treated embryos; WTGH, WT embryos treated with GH; MOGH, tbx5-MO- and GH-treated embryos. Data are presented as the mean ± S.D. *p < 0.05 vs. WT

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
badb WT control Prim-15 whole organism RTPCR
WT + MO1-tbx5a standard conditions Prim-15 whole organism RTPCR
WT + MO1-tbx5a chemical treatment by injection: growth hormone Prim-15 whole organism RTPCR
bcl2a WT control Prim-15 whole organism RTPCR
WT + MO1-tbx5a standard conditions Prim-15 whole organism RTPCR
WT + MO1-tbx5a chemical treatment by injection: growth hormone Prim-15 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-tbx5a standard conditions Prim-15 whole organism badb expression increased amount, abnormal
Prim-15 whole organism bcl2a expression increased amount, abnormal
WT + MO1-tbx5a chemical treatment by injection: growth hormone Prim-15 whole organism badb expression amount, ameliorated
Prim-15 whole organism bcl2a expression amount, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Journal of Biomedical Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Biomed. Sci.