FIGURE

Fig. 6

ID

ZDB-FIG-120525-7

Publication

Mattes et al., 2012 - Wnt3 and Wnt3a are required for induction of the mid-diencephalic organizer in the caudal forebrain

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Fig. 6

Blockage of Wnt signaling lead to lack of MDO tissue. Lateral views and dorsal views (marked by ′) of embryonic heads at 28 hpf. Wnt3/wnt3a morphant embryos lack the organizer tissue and the lhx5 positive prethalamus (PTh, blue) abuts the irx1b thalamus (Th, red) (A-B′, n = 54/78). A similar phenotype is observed in embryos treated with 30 μM IWR1 from 10 to 28 h to inhibit canonical Wnt signaling (n = 15/17). Hence, the otx2 positive MDO (yellow) is lacking if Wnt3/Wnt3a function is knocked-down (n = 44/80, D-E′), likewise after inhibition of canonical Wnt signaling (F, F′, n = 14/24).

Expression Data

Genes:	irx1b lhx5 otx2b
Fish:	WT WT + MO1-wnt3 + MO2-wnt3a
Condition:	chemical treatment: IWR-1-endo
Knockdown Reagents:	MO1-wnt3 MO2-wnt3a
Anatomical Terms:	dorsal thalamus mid diencephalic organizer ventral thalamus
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT WT + MO1-wnt3 + MO2-wnt3a
Condition:	chemical treatment: IWR-1-endo
Knockdown Reagents:	MO1-wnt3 MO2-wnt3a
Observed In:	canonical Wnt signaling pathway diencephalon dorsal thalamus mid diencephalic organizer thalamus development ventral thalamus (all 6)
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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