ZFIN ID: ZDB-FIG-120525-7
Mattes et al., 2012 - Wnt3 and Wnt3a are required for induction of the mid-diencephalic organizer in the caudal forebrain. Neural Development   7(1):12 Full text @ Neural Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 6

Blockage of Wnt signaling lead to lack of MDO tissue. Lateral views and dorsal views (marked by ′) of embryonic heads at 28 hpf. Wnt3/wnt3a morphant embryos lack the organizer tissue and the lhx5 positive prethalamus (PTh, blue) abuts the irx1b thalamus (Th, red) (A-B′, n = 54/78). A similar phenotype is observed in embryos treated with 30 μM IWR1 from 10 to 28 h to inhibit canonical Wnt signaling (n = 15/17). Hence, the otx2 positive MDO (yellow) is lacking if Wnt3/Wnt3a function is knocked-down (n = 44/80, D-E′), likewise after inhibition of canonical Wnt signaling (F, F′, n = 14/24).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
irx1b WT control Prim-5 dorsal thalamus ISH
WT chemical treatment: IWR-1-endo Prim-5 dorsal thalamus ISH
WT + MO1-wnt3 + MO2-wnt3a standard conditions Prim-5 dorsal thalamus ISH
lhx5 WT control Prim-5 ventral thalamus ISH
WT chemical treatment: IWR-1-endo Prim-5 ventral thalamus ISH
WT + MO1-wnt3 + MO2-wnt3a standard conditions Prim-5 ventral thalamus ISH
otx2b WT control Prim-5 dorsal thalamus ISH
Prim-5 mid diencephalic organizer ISH
WT chemical treatment: IWR-1-endo Prim-5 dorsal thalamus ISH
Prim-5 Not Detected mid diencephalic organizer ISH
WT + MO1-wnt3 + MO2-wnt3a standard conditions Prim-5 dorsal thalamus ISH
Prim-5 Not Detected mid diencephalic organizer ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: IWR-1-endo Prim-5 canonical Wnt signaling pathway decreased occurrence, abnormal
Prim-5 diencephalon lacks all parts of type mid diencephalic organizer, abnormal
Prim-5 thalamus development decreased process quality, abnormal
Prim-5 ventral thalamus in contact with dorsal thalamus, abnormal
WT + MO1-wnt3 + MO2-wnt3a standard conditions Prim-5 canonical Wnt signaling pathway decreased occurrence, abnormal
Prim-5 diencephalon lacks all parts of type mid diencephalic organizer, abnormal
Prim-5 thalamus development decreased process quality, abnormal
Prim-5 ventral thalamus in contact with dorsal thalamus, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Neural Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neural Dev.