FIGURE

Fig. 8

ID

ZDB-FIG-120525-9

Publication

Mattes et al., 2012 - Wnt3 and Wnt3a are required for induction of the mid-diencephalic organizer in the caudal forebrain

Other Figures

All Figure Page

Back to All Figure Page

Fig. 8

FezF2 and Irx1b limit the MDO at the AP axis. Shh expression in the MDO is decreased in Wnt3/Wnt3a deficient embryos (A, B, n = 45/55), whereas, prethalamic identity as well as thalamic identity is maintained (E, F, n = 18/23). In triple morphant embryos lacking Wnt3, Wnt3a and Fezf2 function, a restoration of shh expression is observed in the MDO (C, n = 28/31) accompanied by a reduction of lhx5 expression in the pre-thalamus (G, n = 10/34). In wnt3/wnt3a/irx1b triple morphant embryos, we observe a similar restore of the shh positive MDO (D, n = 28/31); however, here the neurog1 positive thalamus shrinks (H, n = 25/32, N, n = 20/25). For explanation of the summary (I-L) see the text.

Expression Data

Genes:	lhx5 neurog1 otx2b shha
Fish:	WT WT + MO1-wnt3 + MO2-wnt3a WT + MO1-fezf2 + MO1-wnt3 + MO2-wnt3a WT + MO1-irx1b + MO1-wnt3 + MO2-wnt3a
Knockdown Reagents:	MO1-fezf2 MO1-irx1b MO1-wnt3 MO2-wnt3a
Anatomical Terms:	dorsal thalamus mid diencephalic organizer ventral thalamus
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-wnt3 + MO2-wnt3a WT + MO1-fezf2 + MO1-wnt3 + MO2-wnt3a WT + MO1-irx1b + MO1-wnt3 + MO2-wnt3a
Knockdown Reagents:	MO1-fezf2 MO1-irx1b MO1-wnt3 MO2-wnt3a
Observed In:	dorsal thalamus mid diencephalic organizer thalamus development ventral thalamus
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Neural Dev.