ZFIN ID: ZDB-FIG-120525-3
Mattes et al., 2012 - Wnt3 and Wnt3a are required for induction of the mid-diencephalic organizer in the caudal forebrain. Neural Development   7(1):12 Full text @ Neural Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 3

Analysis of knock-down of Wnt3 and Wnt3a. Injection of wnt3 MO leads to a decrease in splicing of mRNA at the exon3-exon4 boundary and an increase in non-spliced RNA product visualized by amplification at the exon3-intron3 boundary and subsequently to a reduction of Wnt3 protein in morphant embryos at 28 hpf (A). Knock-down of either Wnt3 or Wnt3a leads to an indistinguishable morphology to the control Morpholino-injected embryos whereas the double morphant embryos show a significant alteration in brain morphology, a kinked tail and a smaller otic vesicle (B, arrows). Similarly, analysis of marker genes in the forebrain midbrain area shows no alteration in control, wnt3 or wnt3a morphant embryos (C-H). Cer, cerebellum.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
irx1b WT control Prim-5 cerebellum ISH
Prim-5 dorsal thalamus ISH
WT + MO1-wnt3 standard conditions Prim-5 cerebellum ISH
Prim-5 dorsal thalamus ISH
WT + MO2-wnt3a standard conditions Prim-5 cerebellum ISH
Prim-5 dorsal thalamus ISH
otx2b WT control Prim-5 dorsal thalamus ISH
Prim-5 mid diencephalic organizer ISH
WT + MO1-wnt3 standard conditions Prim-5 dorsal thalamus ISH
Prim-5 mid diencephalic organizer ISH
WT + MO2-wnt3a standard conditions Prim-5 dorsal thalamus ISH
Prim-5 mid diencephalic organizer ISH
shha WT control Prim-5 mid diencephalic organizer ISH
WT + MO1-wnt3 standard conditions Prim-5 mid diencephalic organizer ISH
WT + MO2-wnt3a standard conditions Prim-5 mid diencephalic organizer ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-wnt3 standard conditions Prim-5 brain morphology, normal
WT + MO1-wnt3 + MO2-wnt3a standard conditions Prim-5 brain malformed, abnormal
Prim-5 otic vesicle decreased size, abnormal
Prim-5 post-vent region kinked, abnormal
WT + MO2-wnt3a standard conditions Prim-5 brain morphology, normal
Acknowledgments:
ZFIN wishes to thank the journal Neural Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neural Dev.