FIGURE

Fig. 3

ID

ZDB-FIG-120525-3

Publication

Mattes et al., 2012 - Wnt3 and Wnt3a are required for induction of the mid-diencephalic organizer in the caudal forebrain

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Fig. 3

Analysis of knock-down of Wnt3 and Wnt3a. Injection of wnt3 MO leads to a decrease in splicing of mRNA at the exon3-exon4 boundary and an increase in non-spliced RNA product visualized by amplification at the exon3-intron3 boundary and subsequently to a reduction of Wnt3 protein in morphant embryos at 28 hpf (A). Knock-down of either Wnt3 or Wnt3a leads to an indistinguishable morphology to the control Morpholino-injected embryos whereas the double morphant embryos show a significant alteration in brain morphology, a kinked tail and a smaller otic vesicle (B, arrows). Similarly, analysis of marker genes in the forebrain midbrain area shows no alteration in control, wnt3 or wnt3a morphant embryos (C-H). Cer, cerebellum.

Expression Data

Genes:	irx1b otx2b shha
Fish:	WT WT + MO1-wnt3 WT + MO2-wnt3a
Knockdown Reagents:	MO1-wnt3 MO2-wnt3a
Anatomical Terms:	cerebellum dorsal thalamus mid diencephalic organizer
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-wnt3 WT + MO2-wnt3a WT + MO1-wnt3 + MO2-wnt3a
Knockdown Reagents:	MO1-wnt3 MO2-wnt3a
Observed In:	brain otic vesicle post-vent region
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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