FIGURE

Fig. 4

ID

ZDB-FIG-120525-4

Publication

Mattes et al., 2012 - Wnt3 and Wnt3a are required for induction of the mid-diencephalic organizer in the caudal forebrain

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Fig. 4

Wnt3 and Wnt3a are required for MDO formation. Knock-down of Wnt3 and Wnt3a leads to a down-regulation of the expression of indicated markers in the central part of the MDO (A-L). Time-lapse analysis of Shh::RFP shows an independent induction of the dorsal remaining organizer spot (M, N). Treatment of wnt3/wnt3a morphant embryos with the Wnt signaling agonist BIO from 10 to 28 h leads to a rescue of shh expression at the MDO (O, P). Brackets mark the extent of the MDO, whereas the arrows indicate the gap between the basal plate and the remaining spot of dorsal MDO identity. Eth, epithlamus, HyTh, hypothalamus, MHB, midbrain-hindbrain boundary, Tel, telencephalon.

Expression Data

Genes:	axin2 DsRed EGFP fgf8a shha shhb wnt8b
Fish:	WT ka300Tg ka300Tg; vu17Tg WT + MO1-wnt3 + MO2-wnt3a ka300Tg + MO1-wnt3 + MO2-wnt3a ka300Tg; vu17Tg + MO1-wnt3 + MO2-wnt3a
Condition:	chemical treatment: 6-bromoindirubin-3'-oxime
Knockdown Reagents:	MO1-wnt3 MO2-wnt3a
Anatomical Terms:	diencephalon posterior region dorsal thalamus dorsal region dorsal thalamus ventral region mid diencephalic organizer mid diencephalic organizer central region mid diencephalic organizer dorsal region mid diencephalic organizer ventral region midbrain hindbrain boundary
Stage Range:	Prim-5 to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-wnt3 + MO2-wnt3a ka300Tg; vu17Tg + MO1-wnt3 + MO2-wnt3a
Knockdown Reagents:	MO1-wnt3 MO2-wnt3a
Observed In:	canonical Wnt signaling pathway mid diencephalic organizer midbrain hindbrain boundary thalamus development
Stage Range:	Prim-5 to Prim-25

Phenotype Detail

Acknowledgments

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