ZFIN ID: ZDB-FIG-120525-4
Mattes et al., 2012 - Wnt3 and Wnt3a are required for induction of the mid-diencephalic organizer in the caudal forebrain. Neural Development   7(1):12 Full text @ Neural Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Prim-25
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Prim-25

Fig. 4

Wnt3 and Wnt3a are required for MDO formation. Knock-down of Wnt3 and Wnt3a leads to a down-regulation of the expression of indicated markers in the central part of the MDO (A-L). Time-lapse analysis of Shh::RFP shows an independent induction of the dorsal remaining organizer spot (M, N). Treatment of wnt3/wnt3a morphant embryos with the Wnt signaling agonist BIO from 10 to 28 h leads to a rescue of shh expression at the MDO (O, P). Brackets mark the extent of the MDO, whereas the arrows indicate the gap between the basal plate and the remaining spot of dorsal MDO identity. Eth, epithlamus, HyTh, hypothalamus, MHB, midbrain-hindbrain boundary, Tel, telencephalon.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
axin2 WT control Prim-5 diencephalon posterior region ISH
WT + MO1-wnt3 + MO2-wnt3a standard conditions Prim-5 diencephalon posterior region ISH
DsRed ka300Tg control Prim-5 mid diencephalic organizer central region IFL
Prim-5 mid diencephalic organizer ventral region IFL
Prim-15 to Prim-25 mid diencephalic organizer central region IFL
Prim-15 to Prim-25 mid diencephalic organizer dorsal region IFL
Prim-15 to Prim-25 mid diencephalic organizer ventral region IFL
ka300Tg + MO1-wnt3 + MO2-wnt3a standard conditions Prim-5 Not Detected mid diencephalic organizer central region IFL
Prim-5 mid diencephalic organizer dorsal region IFL
Prim-5 mid diencephalic organizer ventral region IFL
Prim-15 to Prim-25 Not Detected mid diencephalic organizer central region IFL
Prim-15 to Prim-25 mid diencephalic organizer dorsal region IFL
Prim-15 to Prim-25 mid diencephalic organizer ventral region IFL
ka300Tg; vu17Tg control Prim-25 mid diencephalic organizer central region IFL
Prim-25 mid diencephalic organizer dorsal region IFL
Prim-25 mid diencephalic organizer ventral region IFL
ka300Tg; vu17Tg + MO1-wnt3 + MO2-wnt3a standard conditions Prim-25 Not Detected mid diencephalic organizer central region IFL
Prim-25 mid diencephalic organizer dorsal region IFL
Prim-25 mid diencephalic organizer ventral region IFL
EGFP ka300Tg; vu17Tg control Prim-25 dorsal thalamus dorsal region IFL
Prim-25 dorsal thalamus ventral region IFL
ka300Tg; vu17Tg + MO1-wnt3 + MO2-wnt3a standard conditions Prim-25 dorsal thalamus dorsal region IFL
Prim-25 Not Detected dorsal thalamus ventral region IFL
fgf8a WT control Prim-5 midbrain hindbrain boundary ISH
Prim-25 midbrain hindbrain boundary ISH
WT + MO1-wnt3 + MO2-wnt3a standard conditions Prim-5 Not Detected midbrain hindbrain boundary ISH
Prim-25 Not Detected midbrain hindbrain boundary ISH
shha WT control Prim-5 mid diencephalic organizer central region ISH
Prim-5 mid diencephalic organizer ventral region ISH
Prim-25 mid diencephalic organizer central region ISH
Prim-25 mid diencephalic organizer dorsal region ISH
Prim-25 mid diencephalic organizer ventral region ISH
WT + MO1-wnt3 + MO2-wnt3a chemical treatment: 6-bromoindirubin-3'-oxime Prim-5 mid diencephalic organizer central region ISH
Prim-5 mid diencephalic organizer dorsal region ISH
Prim-5 mid diencephalic organizer ventral region ISH
WT + MO1-wnt3 + MO2-wnt3a standard conditions Prim-5 Not Detected mid diencephalic organizer central region ISH
Prim-5 mid diencephalic organizer ventral region ISH
Prim-25 Not Detected mid diencephalic organizer central region ISH
Prim-25 mid diencephalic organizer dorsal region ISH
Prim-25 mid diencephalic organizer ventral region ISH
shhb WT control Prim-5 mid diencephalic organizer ISH
WT + MO1-wnt3 + MO2-wnt3a standard conditions Prim-5 Not Detected mid diencephalic organizer ISH
wnt8b WT control Prim-5 mid diencephalic organizer central region ISH
Prim-5 mid diencephalic organizer dorsal region ISH
Prim-5 mid diencephalic organizer ventral region ISH
WT + MO1-wnt3 + MO2-wnt3a standard conditions Prim-5 Not Detected mid diencephalic organizer central region ISH
Prim-5 mid diencephalic organizer dorsal region ISH
Prim-5 mid diencephalic organizer ventral region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-wnt3 + MO2-wnt3a standard conditions Prim-5 canonical Wnt signaling pathway decreased occurrence, abnormal
Prim-5 mid diencephalic organizer cellular quality, abnormal
Prim-5 midbrain hindbrain boundary cellular quality, abnormal
Prim-5 thalamus development decreased process quality, abnormal
Prim-25 canonical Wnt signaling pathway decreased occurrence, abnormal
Prim-25 mid diencephalic organizer cellular quality, abnormal
Prim-25 midbrain hindbrain boundary cellular quality, abnormal
Prim-25 thalamus development decreased process quality, abnormal
ka300Tg; vu17Tg + MO1-wnt3 + MO2-wnt3a standard conditions Prim-25 canonical Wnt signaling pathway decreased occurrence, abnormal
Prim-25 mid diencephalic organizer cellular quality, abnormal
Prim-25 thalamus development decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Neural Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neural Dev.