ZFIN ID: ZDB-FIG-120224-3
Mullins et al., 1996 - Genes establishing dorsoventral pattern formation in the zebrafish embryo: the ventral specifying genes. Development (Cambridge, England)   123:81-93 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Shield to 10-13 somites
PHENOTYPE:
Fish:
Observed In:
Stage Range: 5-9 somites to 10-13 somites

Fig. 4

Altered expression of gata1, eve1, fkd3 and pax2 in whole-mount in situ hybridizations of the dorsalized mutants. Expression of gata1 in 8-somite stage wild-type embryos (A,B), swr (C,D), snh (E,F,G), pgy (H), and laf (I) mutant embryos. Double stainings with gata1 and anti-Ntl antibody are shown as whole mounts in A,C,E. Spreads are shown in (B,D,F-I). eve1, gsc double staining at shield stage in wild-type (J) and swr mutant (K) embryos (white arrowheads delineate gsc expression). eve1, gsc double stainings at 70% epiboly in wild-type (L), and snh mutant (M) embryos (white arrow marks gsc expression). fkd3 expression at 70% epiboly in wild-type (N), swr mutant (O), and snh mutant (P) embryos (black arrows indicate the lateral extent of staining). Spreads of pax2-stained 10-somite stage wild type (Q), swr (the most anterior position of the embryo lies in the middle of the ring; R), snh (S), pgy (T), and laf (U) mutant embryos. op, optic vesicle and stalk; mb, midbrain; ot, otic vesicle; n, neuronal and p, pronephric precursor expression domains.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
eve1 TU standard conditions Shield margin ISH
bmp2btc300a/tc300a (TU) standard conditions Shield margin ISH
bmp7aty68a/ty68a standard conditions Shield margin ISH
foxb1a TU standard conditions Shield presumptive neural plate ISH
bmp2bta72a/ta72a (TU) standard conditions Shield presumptive neural plate ISH
bmp7aty68a/ty68a standard conditions Shield presumptive neural plate ISH
gata1a TU standard conditions 5-9 somites lateral mesoderm ISH
acvr1ltm110b/tm110b (TU) standard conditions 5-9 somites lateral mesoderm ISH
bmp2bta72a/ta72a (TU) standard conditions 5-9 somites Not Detected lateral mesoderm ISH
bmp7aty68a/ty68a standard conditions 5-9 somites Not Detected lateral mesoderm ISH
smad5dty40/dty40 (TU) standard conditions 5-9 somites lateral mesoderm ISH
gsc TU standard conditions Shield shield ISH
bmp2btc300a/tc300a (TU) standard conditions Shield shield ISH
bmp7aty68a/ty68a standard conditions Shield shield ISH
pax2a TU standard conditions 10-13 somites hindbrain neuron ISH
10-13 somites midbrain ISH
10-13 somites optic stalk ISH
10-13 somites optic vesicle ISH
10-13 somites otic placode ISH
10-13 somites pronephric duct ISH
10-13 somites spinal cord neural rod neuron ISH
acvr1ltm110b/tm110b (TU) standard conditions 10-13 somites hindbrain neuron ISH
10-13 somites midbrain ISH
10-13 somites optic stalk ISH
10-13 somites optic vesicle ISH
10-13 somites otic placode ISH
10-13 somites pronephric duct ISH
10-13 somites spinal cord neural rod neuron ISH
bmp2bta72a/ta72a (TU) standard conditions 10-13 somites midbrain ISH
10-13 somites optic vesicle ISH
bmp7aty68a/ty68a standard conditions 10-13 somites midbrain ISH
10-13 somites optic vesicle ISH
10-13 somites pronephric duct ISH
10-13 somites spinal cord neural rod neuron ISH
smad5dty40/dty40 (TU) standard conditions 10-13 somites hindbrain neuron ISH
10-13 somites midbrain ISH
10-13 somites optic vesicle ISH
10-13 somites otic placode ISH
10-13 somites pronephric duct ISH
10-13 somites spinal cord neural rod neuron ISH
tbxta TU standard conditions 5-9 somites notochord ISH
bmp2bta72a/ta72a (TU) standard conditions 5-9 somites notochord ISH
bmp7aty68a/ty68a standard conditions 5-9 somites notochord ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
acvr1ltm110b/tm110b (TU) standard conditions 5-9 somites erythroid lineage cell decreased amount, abnormal
bmp2bta72a/ta72a (TU) standard conditions 5-9 somites erythroid lineage cell absent, abnormal
10-13 somites neural tube morphology, abnormal
10-13 somites optic vesicle mislocalised, abnormal
10-13 somites otic placode aplastic, abnormal
10-13 somites pronephric duct aplastic, abnormal
10-13 somites spinal cord neural rod neuron absent, abnormal
bmp7aty68a/ty68a standard conditions 5-9 somites erythroid lineage cell absent, abnormal
10-13 somites neural tube morphology, abnormal
10-13 somites otic placode aplastic, abnormal
10-13 somites pronephric duct hypoplastic, abnormal
10-13 somites spinal cord neural rod neuron increased amount, abnormal
10-13 somites spinal cord neural rod neuron mislocalised laterally, abnormal
smad5dty40/dty40 (TU) standard conditions 5-9 somites erythroid lineage cell decreased amount, abnormal
10-13 somites pronephric duct decreased length, abnormal
10-13 somites pronephros increased width, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development