ZFIN ID: ZDB-FIG-120224-3
Mullins et al., 1996 - Genes establishing dorsoventral pattern formation in the zebrafish embryo: the ventral specifying genes. Development (Cambridge, England)   123:81-93
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Shield to 10-13 somites
PHENOTYPE:
Fish:
Observed In:
Stage Range: 5-9 somites to 10-13 somites

Fig. 4

Altered expression of gata1, eve1, fkd3 and pax2 in whole-mount in situ hybridizations of the dorsalized mutants. Expression of gata1 in 8-somite stage wild-type embryos (A,B), swr (C,D), snh (E,F,G), pgy (H), and laf (I) mutant embryos. Double stainings with gata1 and anti-Ntl antibody are shown as whole mounts in A,C,E. Spreads are shown in (B,D,F-I). eve1, gsc double staining at shield stage in wild-type (J) and swr mutant (K) embryos (white arrowheads delineate gsc expression). eve1, gsc double stainings at 70% epiboly in wild-type (L), and snh mutant (M) embryos (white arrow marks gsc expression). fkd3 expression at 70% epiboly in wild-type (N), swr mutant (O), and snh mutant (P) embryos (black arrows indicate the lateral extent of staining). Spreads of pax2-stained 10-somite stage wild type (Q), swr (the most anterior position of the embryo lies in the middle of the ring; R), snh (S), pgy (T), and laf (U) mutant embryos. op, optic vesicle and stalk; mb, midbrain; ot, otic vesicle; n, neuronal and p, pronephric precursor expression domains.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
eve1 TU standard conditions Shield margin ISH
bmp2btc300a/tc300a(TU) standard conditions Shield margin ISH
bmp7aty68a/ty68a standard conditions Shield margin ISH
foxb1a TU standard conditions Shield presumptive neural plate ISH
bmp2bta72a/ta72a(TU) standard conditions Shield presumptive neural plate ISH
bmp7aty68a/ty68a standard conditions Shield presumptive neural plate ISH
gata1a TU standard conditions 5-9 somites lateral mesoderm ISH
acvr1ltm110b/tm110b(TU) standard conditions 5-9 somites lateral mesoderm ISH
bmp2bta72a/ta72a(TU) standard conditions 5-9 somites Not Detected lateral mesoderm ISH
bmp7aty68a/ty68a standard conditions 5-9 somites Not Detected lateral mesoderm ISH
smad5dty40/dty40(TU) standard conditions 5-9 somites lateral mesoderm ISH
gsc TU standard conditions Shield shield ISH
bmp2btc300a/tc300a(TU) standard conditions Shield shield ISH
bmp7aty68a/ty68a standard conditions Shield shield ISH
pax2a TU standard conditions 10-13 somites hindbrain neuron ISH
10-13 somites midbrain ISH
10-13 somites optic stalk ISH
10-13 somites optic vesicle ISH
10-13 somites otic placode ISH
10-13 somites pronephric duct ISH
10-13 somites spinal cord neural rod neuron ISH
acvr1ltm110b/tm110b(TU) standard conditions 10-13 somites hindbrain neuron ISH
10-13 somites midbrain ISH
10-13 somites optic stalk ISH
10-13 somites optic vesicle ISH
10-13 somites otic placode ISH
10-13 somites pronephric duct ISH
10-13 somites spinal cord neural rod neuron ISH
bmp2bta72a/ta72a(TU) standard conditions 10-13 somites midbrain ISH
10-13 somites optic vesicle ISH
bmp7aty68a/ty68a standard conditions 10-13 somites midbrain ISH
10-13 somites optic vesicle ISH
10-13 somites pronephric duct ISH
10-13 somites spinal cord neural rod neuron ISH
smad5dty40/dty40(TU) standard conditions 10-13 somites hindbrain neuron ISH
10-13 somites midbrain ISH
10-13 somites optic vesicle ISH
10-13 somites otic placode ISH
10-13 somites pronephric duct ISH
10-13 somites spinal cord neural rod neuron ISH
tbxta TU standard conditions 5-9 somites notochord ISH
bmp2bta72a/ta72a(TU) standard conditions 5-9 somites notochord ISH
bmp7aty68a/ty68a standard conditions 5-9 somites notochord ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
acvr1ltm110b/tm110b(TU) standard conditions 5-9 somites erythroid lineage cell decreased amount, abnormal
bmp2bta72a/ta72a(TU) standard conditions 5-9 somites erythroid lineage cell absent, abnormal
10-13 somites neural tube morphology, abnormal
10-13 somites optic vesicle mislocalised, abnormal
10-13 somites otic placode aplastic, abnormal
10-13 somites pronephric duct aplastic, abnormal
10-13 somites spinal cord neural rod neuron absent, abnormal
bmp7aty68a/ty68a standard conditions 5-9 somites erythroid lineage cell absent, abnormal
10-13 somites neural tube morphology, abnormal
10-13 somites otic placode aplastic, abnormal
10-13 somites pronephric duct hypoplastic, abnormal
10-13 somites spinal cord neural rod neuron increased amount, abnormal
10-13 somites spinal cord neural rod neuron mislocalised laterally, abnormal
smad5dty40/dty40(TU) standard conditions 5-9 somites erythroid lineage cell decreased amount, abnormal
10-13 somites pronephric duct decreased length, abnormal
10-13 somites pronephros increased width, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.