ZFIN ID: ZDB-FIG-120224-2
Mullins et al., 1996 - Genes establishing dorsoventral pattern formation in the zebrafish embryo: the ventral specifying genes. Development (Cambridge, England)   123:81-93
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Bud to 14-19 somites
PHENOTYPE:
Fish:
Observed In:
Stage Range: Bud to 14-19 somites

Fig. 3

Ntl and myoD expression in swr, snh, pgy and laf mutant embryos. Animal pole or anterior is left or up, except where noted. A dorsal view of whole-mount anti- Ntl antibody stainings at the bud stage of wild type (A); swr (B) where the Ntl notochord domain is about twice as broad as in wild type; snh (C) where the Ntl domain is about 50% broader than in wild type; and pgy (D) where the Ntl domain is about 25% broader than in wild type. In EH, S,T the yolk was removed and then the axis of the embryo flattened onto a slide in order to visualize the entire axis of the embryo (a ‘spread’). Spreads of myoD in situ hybridizations of bud stage embryos in wild type (E,E′), swr (F,F′), snh (G,G′), and pgy (H,H′) mutant embryos. Higher magnifications of the posterior expression (E′-H′) show the mediolateral expansion of myoD expression. Lateral view of a whole-mount myoD staining at the 18- somite stage in wild-type (I) and laf mutant (J) embryos. An arrowhead indicates the enlarged somitic expression of myoD in laf. Dorsal (K,M,Q) and lateral (L,N,R) views of whole-mount myoD stainings of wild-type, swr and snh embryos at the 6-7 somite stage. From a dorsal view of snh (Q) 7 somites are visible. In a lateral view of the same snh embryo (R), it is apparent that only the 5 most posterior somites extend around the circumference of the embryo. Spreads of myoD staining at the 5-6 somite stage in wild type (S) and pgy (T). The wild-type embryo (K) is shown from the vegetal pole (O). In P a vegetal pole view of the swr embryo in M shows the first two somites extending around the circumference of the embryo. The more posterior somites also extend to the ventral side and fuse, but are not in the plane of focus.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myod1 TU standard conditions Bud adaxial cell ISH
Bud paraxial mesoderm ISH
5-9 somites segmental plate ISH
5-9 somites somite ISH
acvr1ltm110b/tm110b(TU) standard conditions 14-19 somites somite ISH
bmp2bta72a/ta72a(TU) standard conditions 5-9 somites segmental plate ISH
5-9 somites somite ISH
bmp2btc300a/tc300a(TU) standard conditions Bud adaxial cell ISH
Bud paraxial mesoderm ISH
Bud tail bud ISH
bmp7aty68a/ty68a standard conditions Bud adaxial cell ISH
Bud paraxial mesoderm ISH
Bud tail bud ISH
5-9 somites segmental plate ISH
5-9 somites somite ISH
smad5dty40/dty40(TU) standard conditions Bud adaxial cell ISH
Bud paraxial mesoderm ISH
Bud tail bud ISH
5-9 somites segmental plate ISH
5-9 somites somite ISH
tbxta TU standard conditions Bud notochord ISH
bmp2bta72a/ta72a(TU) standard conditions Bud notochord ISH
bmp7aty68a/ty68a standard conditions Bud notochord ISH
smad5dty40/dty40(TU) standard conditions Bud notochord ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
acvr1ltm110b/tm110b(TU) standard conditions 14-19 somites post-vent region somite increased width, abnormal
bmp2bta72a/ta72a(TU) standard conditions Bud notochord increased width, abnormal
Bud somite fused with somite, abnormal
bmp2btc300a/tc300a(TU) standard conditions Bud paraxial mesoderm anterior-posterior axis decreased length, abnormal
Bud paraxial mesoderm has extra parts of type adaxial cell, abnormal
bmp7aty68a/ty68a standard conditions Bud notochord increased width, abnormal
Bud paraxial mesoderm anterior-posterior axis decreased length, abnormal
Bud paraxial mesoderm has extra parts of type adaxial cell, abnormal
Bud somite increased width, abnormal
smad5dty40/dty40(TU) standard conditions Bud notochord increased width, abnormal
Bud paraxial mesoderm anterior-posterior axis decreased length, abnormal
Bud paraxial mesoderm has extra parts of type adaxial cell, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.