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Fig. S1 Comparison of human, mouse and zebrafish Grhl2 orthologs. (A) Amino acid alignment of Grhl2 from human (hGrhl2), mouse (mGrhl2) and zebrafish (zGrhl2a;zGrhl2b); boxed regions highlight highly conserved functional domains (TA, transactivation domain; DBD, DNA-binding domain; DIM, dimerisation domain). (B) Table showing the percentage identity and homology between human, mouse and zebrafish grhl2a and grhl2b sequences at the amino acid level. (C-G) Analysis of zebrafish grhl2b mRNA expression by in situ hybridisation from 8 hpf to 24 hpf. p, polster; anp and lnp, anterior and lateral neural plate; bp, brain primordium; nc, neural crest, ov, presumptive otic vesicles; op, optic primordium; oe, olfactory epithelium; pm, pronephric mesoderm. Expression is seen in the neural crest, both adaxially and ventrally to the neural tube (inset, nt, sagittal view) and otic vesicles. Flat-mount, dorsal view at 24 hpf (G) shows persistent expression in the olfactory epithelium, otic vesicles and neural crest.