FIGURE

Fig. 9

ID

ZDB-FIG-120126-85

Publication

Neto et al., 2012 - The osr1 and osr2 genes act in the pronephric anlage downstream of retinoic acid signaling and upstream of wnt2b to maintain pectoral fin development

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Fig. 9

Temporal requirement of RA for tbx5a, wnt2b and Osr expression. Dorsal views of embryos at the 21- to 24-somite stage treated with DEAB from the time shown above the panels showing the expression of genes indicated on the left. (A,E,I,M,Q) Control embryos incubated with DMSO. (B,F,J,N,R) Interference with RA signaling from 50% epiboly caused strong downregulation of all genes examined. (C,G,K,O,S) Incubation with DEAB from tailbud caused strong downregulation of tbx5a, osr1, wnt2ba and wnt2bb (C,G,O,S, respectively) while not affecting osr2 expression (K). (D,H,L,P,T) Culturing embryos with DEAB from the 6-somite stage only caused some tbx5a downregulation (D). Arrowheads indicate the tbx5a-expressing domain (A-D) and the kidney anlage (E-T).

Expression Data

Genes:	osr1 osr2 tbx5a wnt2ba wnt2bb
Fish:	WT
Condition:	chemical treatment: pharmaceutical
Anatomical Terms:	intermediate mesoderm pectoral fin field pronephros
Stage:	20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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