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ZFIN ID: ZDB-FIG-120126-82
Neto et al., 2012 - The osr1 and osr2 genes act in the pronephric anlage downstream of retinoic acid signaling and upstream of wnt2b to maintain pectoral fin development. Development (Cambridge, England)   139(2):301-11 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage Range: 10-13 somites to 26+ somites

Fig. 6

wnt2b expression in the intermediate mesoderm requires Osr function. mRNA in situ hybridizations on 13-somite stage (wnt2ba and tbx5a) and 22 hpf (wnt2bb) embryos using riboprobes as indicated on the left. All panels show dorsal views with anterior towards the left. (A-H) wnt2ba (A-D) and wnt2bb (E-H) expression in the IM (arrowheads) in wild-type (A,E), single (B,C,F,G) and double (D,H) Osr-morphant embryos. (I-L) Increasing Wnt signaling in double Osr-morphant embryos rescues tbx5a expression in the limb buds at 13 somites. (I,J) Embryos injected with β-catenin-GR mRNA show normal (I) or slightly larger (J) tbx5a expression domains (arrowheads) in the absence or presence of dexamethasone, respectively. (K,L) Embryos co-injected with β-catenin-GR mRNA and the two Osr MOs show reduced (K, arrowhead) or rescued (L, arrowhead) tbx5a expression domains in the absence or presence of dexamethasone, respectively. hpf, hours postfertilization; s, somite.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
wnt2ba WT control 10-13 somites intermediate mesoderm ISH
WT + MO1-osr1 standard conditions 10-13 somites intermediate mesoderm ISH
WT + MO1-osr1 + MO1-osr2 standard conditions 10-13 somites intermediate mesoderm ISH
WT + MO1-osr2 standard conditions 10-13 somites intermediate mesoderm ISH
wnt2bb WT control 26+ somites intermediate mesoderm ISH
WT + MO1-osr1 standard conditions 26+ somites intermediate mesoderm ISH
WT + MO1-osr1 + MO1-osr2 standard conditions 26+ somites intermediate mesoderm ISH
WT + MO1-osr2 standard conditions 26+ somites intermediate mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development