ZFIN ID: ZDB-FIG-111220-13
Kimmel et al., 2011 - Requirement for Pdx1 in specification of latent endocrine progenitors in zebrafish. BMC Biology   9(1):75 Full text @ BMC Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 3 Absence of ventral bud endocrine precursors in hb9/pdx1 and pdx1 morphant embryos. (A-D) Projections of confocal stacks showing native fluorescence of 3 days post fertilization (dpf) TgBAC(NeuroD:EGFP)nl1; Tg(ins:dsRed)m1018 embryos. In uninjected control embryos (A) and in hb9 morphants (C), enhanced green fluorescent protein (EGFP)+ cells are found in the islet and in smaller numbers also anterior to the islet. In hb9/pdx1 double morphants (B) and pdx1 morphants (D), the number of islet-associated EGFP+ cells is strongly reduced and anterior cells are missing. Few Ins:DsRed cells are present in pdx1 single morphants at 3 dpf (D), reflecting slow maturation of the DsRed fluorophore. (E) Schematic of pancreas morphology at 3 dpf. The principal islet (Isl) at this stage is primarily dorsal bud derived. The ventral bud contributes new NeuroD:EGFP cells (green circles), and generates exocrine pancreas (Exo) and extrapancreatic duct (EPD). IB, intestinal bulb; SB, swim bladder. Red box delineates region included for quantitation of islet and newly emerging anterior endocrine cells. (F) Quantitation of pancreatic EGFP+ cells (*P < 0.05, ***P < 0.001 with P values determined using one-way analysis of variance (ANOVA) with Bonferroni′s post test). (G) Quantitation of anteriorly positioned EGFP+ cells in uninjected and morpholino injected embryos (error bars indicate standard deviation from the mean). Scale bar = 10 μM.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
DsRed m1018Tg; nl1Tg control Protruding-mouth extrapancreatic duct IFL
Protruding-mouth pancreatic B cell IFL
m1018Tg; nl1Tg + MO1-pdx1 standard conditions Protruding-mouth pancreatic B cell IFL
m1018Tg; nl1Tg + MO1-pdx1 + MO2-mnx1 standard conditions Protruding-mouth Not Detected pancreatic B cell IFL
m1018Tg; nl1Tg + MO2-mnx1 standard conditions Protruding-mouth extrapancreatic duct IFL
Protruding-mouth pancreatic B cell IFL
EGFP m1018Tg; nl1Tg control Protruding-mouth endocrine pancreas endoderm IFL
m1018Tg; nl1Tg + MO1-pdx1 standard conditions Protruding-mouth endocrine pancreas endoderm IFL
m1018Tg; nl1Tg + MO1-pdx1 + MO2-mnx1 standard conditions Protruding-mouth endocrine pancreas endoderm IFL
m1018Tg; nl1Tg + MO2-mnx1 standard conditions Protruding-mouth endocrine pancreas endoderm IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
m1018Tg; nl1Tg + MO1-pdx1 standard conditions Protruding-mouth endocrine pancreas decreased size, abnormal
Protruding-mouth endocrine pancreas development disrupted, abnormal
Protruding-mouth endocrine pancreas has fewer parts of type endodermal cell, abnormal
Protruding-mouth pancreatic B cell decreased amount, abnormal
Protruding-mouth type B pancreatic cell differentiation disrupted, abnormal
Protruding-mouth type B pancreatic cell proliferation disrupted, abnormal
m1018Tg; nl1Tg + MO1-pdx1 + MO2-mnx1 standard conditions Protruding-mouth endocrine pancreas decreased size, abnormal
Protruding-mouth endocrine pancreas has fewer parts of type endodermal cell, abnormal
Protruding-mouth pancreatic B cell absent, abnormal
Protruding-mouth type B pancreatic cell differentiation disrupted, abnormal
Protruding-mouth type B pancreatic cell proliferation disrupted, abnormal
m1018Tg; nl1Tg + MO2-mnx1 standard conditions Protruding-mouth endocrine pancreas development disrupted, abnormal
Protruding-mouth pancreatic B cell decreased amount, abnormal
Protruding-mouth type B pancreatic cell differentiation disrupted, abnormal
Protruding-mouth type B pancreatic cell proliferation disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal BMC Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Biol.