ZFIN ID: ZDB-FIG-111220-12
Kimmel et al., 2011 - Requirement for Pdx1 in specification of latent endocrine progenitors in zebrafish. BMC Biology   9(1):75 Full text @ BMC Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 2 Disrupted precursor specification and differentiation in hb9/pdx1 double morphants. (A,B) Projections of confocal image stacks of native enhanced green fluorescent protein (EGFP) expression in control uninjected and in hb9/pdx1 morpholino injected TgBAC(NeuroD:EGFP)nl1 embryos at 28 h post fertilization (hpf). In morphants, the clustering of EGFP labeled cells is disrupted (B) and the number of EGFP expressing cells is reduced as compared to controls (C). By 52 hpf, NeuroD:EGFP cell numbers have increased in control embryos, but not changed in the morphants ((C), *P < 0.05, ***P < 0.001 as determined by one-way analysis of variance (ANOVA) with Bonferroni′s post test). (D) Schematic depicting overall gut morphology in 52 hpf embryos. EPD, extrapancreatic duct; IB, intestinal bulb; ISL, islet; SB, swim bladder. (E,F) Confocal image projections of control uninjected (E) and hb9/pdx1 morpholino injected (F) TgBAC(NeuroD:EGFP)nl1;Tg(ins:dsRed)m1018 embryos at 52 hpf that were costained with antibodies against pan-cadherin (light blue) to outline epithelial structures. In control embryos, EGFP+ and EGFP+/DsRed+ cells are mainly found in the islet. A rare EGFP+/DsRed+ cell is seen in a distal location (E, arrow). In double morphants, few to no EGFP/DsRed-expressing cells can be detected and many EGFP+ cells are located at ectopic positions outside the developing gut epithelium (F). Scale bar = 15 μM in all panels. All embryos are shown from ventral view, anterior to the left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
DsRed m1018Tg; nl1Tg control Long-pec endocrine pancreas IFL
Long-pec pancreatic B cell IFL
m1018Tg; nl1Tg + MO1-pdx1 + MO2-mnx1 standard conditions Long-pec Not Detected pancreatic B cell IFL
EGFP m1018Tg; nl1Tg control Long-pec endocrine pancreas IFL
m1018Tg; nl1Tg + MO1-pdx1 + MO2-mnx1 standard conditions Long-pec endocrine pancreas IFL
nl1Tg control Prim-5 posterior pancreatic bud endoderm IFL
nl1Tg + MO1-pdx1 + MO2-mnx1 standard conditions Prim-5 posterior pancreatic bud endoderm IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-pan-Cadherin IHC m1018Tg; nl1Tg control Long-pec endocrine pancreas
IHC Long-pec extrapancreatic duct
IHC Long-pec intestinal bulb
IHC Long-pec swim bladder bud
IHC m1018Tg; nl1Tg + MO1-pdx1 + MO2-mnx1 standard conditions Long-pec endocrine pancreas
IHC Long-pec extrapancreatic duct
IHC Long-pec intestinal bulb
IHC Long-pec swim bladder bud
Phenotype Details
Fish Conditions Stage Phenotype
m1018Tg; nl1Tg + MO1-pdx1 + MO2-mnx1 standard conditions Long-pec endocrine pancreas development disrupted, abnormal
Long-pec pancreatic B cell absent, abnormal
Long-pec type B pancreatic cell differentiation absent, abnormal
nl1Tg + MO1-pdx1 + MO2-mnx1 standard conditions Prim-5 endocrine pancreas development disrupted, abnormal
Prim-5 posterior pancreatic bud cellular motility, abnormal
Prim-5 posterior pancreatic bud elongated, abnormal
Acknowledgments:
ZFIN wishes to thank the journal BMC Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Biol.