ZFIN ID: ZDB-FIG-111214-27
Teng et al., 2011 - Loss of Zebrafish lgi1b Leads to Hydrocephalus and Sensitization to Pentylenetetrazol Induced Seizure-Like Behavior. PLoS One   6(9):e24596 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage Range: Prim-5 to Protruding-mouth

Fig. 2 Splice-site-targeted morpholino oligonucleotides alter normal lgi1b splicing. (A) A schematic diagram of the partial pre-mRNA map showing the predicted outcomes in lgi1b morphants (left panel). The exons are shown in boxes, labeled with the corresponding exon number E1–E3, and the introns are represented by solid lines. The location of the exon 2 splice site, targeted by the MO-E2 morpholino, and the primer binding sites (p1, p2, p3, arrows) for PCR validation are indicated. Semi-qRT-PCR analysis (right panel) of lgi1b transcript levels in wild-type fish, MO-E2mis and MO-E2 morphants at different time points (48 and 72 hpf) show aberrant splicing in the MO-E2 (2 ng) morphants which generates an abnormal (251 bp) mRNA (lacking exon 2) using primers p1/p3. (B) qRT-PCR analysis of lgi1b transcript levels in wild-type, MO-E2mis and MO-E2 morphants following injection of three different concentrations of MO-E2 (2, 3 and 4 ng). (C) Semi-qRT-PCR analysis of transcript levels of the zebrafish lgi1 family members after knockdown of lgi1a (left) and lgi1b (right) respectively.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
lgi1a WT control Prim-5 whole organism RTPCR
Protruding-mouth whole organism RTPCR
WT + MO1-lgi1b standard conditions Prim-5 whole organism RTPCR
Protruding-mouth whole organism RTPCR
WT + MO2-lgi1a standard conditions Prim-5 whole organism RTPCR
Protruding-mouth Not Detected whole organism RTPCR
lgi1b WT control Prim-5 whole organism RTPCR
Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
WT standard conditions Long-pec whole organism RTPCR
WT + MO1-lgi1b standard conditions Prim-5 whole organism RTPCR
Long-pec whole organism RTPCR
Protruding-mouth Not Detected whole organism RTPCR
WT + MO2-lgi1a standard conditions Prim-5 whole organism RTPCR
Protruding-mouth whole organism RTPCR
lgi2a WT control Prim-5 whole organism RTPCR
Protruding-mouth whole organism RTPCR
WT + MO1-lgi1b standard conditions Prim-5 whole organism RTPCR
Protruding-mouth whole organism RTPCR
WT + MO2-lgi1a standard conditions Prim-5 whole organism RTPCR
Protruding-mouth whole organism RTPCR
lgi2b WT control Prim-5 whole organism RTPCR
Protruding-mouth whole organism RTPCR
WT + MO1-lgi1b standard conditions Prim-5 whole organism RTPCR
Protruding-mouth whole organism RTPCR
WT + MO2-lgi1a standard conditions Prim-5 whole organism RTPCR
Protruding-mouth whole organism RTPCR
lgi3 WT control Prim-5 whole organism RTPCR
Protruding-mouth whole organism RTPCR
WT + MO1-lgi1b standard conditions Prim-5 whole organism RTPCR
Protruding-mouth whole organism RTPCR
WT + MO2-lgi1a standard conditions Prim-5 whole organism RTPCR
Protruding-mouth whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One