ZFIN ID: ZDB-FIG-111214-29
Teng et al., 2011 - Loss of Zebrafish lgi1b Leads to Hydrocephalus and Sensitization to Pentylenetetrazol Induced Seizure-Like Behavior. PLoS One   6(9):e24596 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 6 Analysis of c-fos expression as a marker for seizure-like behavior. Increased expression of c-fos in lgi1a (MO-E3), but not lgi1b (MO-E2), morphants compared with corresponding mismatch (mis) MO treatment. RNA and protein extracts from 3 dpf embryos injected with 3 ng of the indicated morpholinos or wildtype were used for semi-qRT-PCR (A) and western blot assays (B), respectively. When lgi1 morphants (MO-E2) were treated with PTZ, an increase in c-fos expression was observed. No increase in c-fos was seen when mismatch morphants (MO-E3mis) were treated with PTZ.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
fosab WT control Protruding-mouth whole organism RTPCR
Protruding-mouth whole organism WB
WT standard conditions Protruding-mouth whole organism WB
Protruding-mouth whole organism RTPCR
WT + MO1-lgi1b standard conditions Protruding-mouth whole organism RTPCR
Protruding-mouth whole organism WB
WT + MO2-lgi1a standard conditions Protruding-mouth whole organism WB
Protruding-mouth whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-lgi1a standard conditions Protruding-mouth regulation of transmission of nerve impulse decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One