FIGURE

Fig. 6

ID

ZDB-FIG-111128-22

Publication

Choe et al., 2011 - A screen for hoxb1-regulated genes identifies ppp1r14al as a regulator of the rhombomere 4 Fgf-signaling center

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Disruption of ppp1r14al leads to loss of fgf3 expression. (A–N) Control (A, C, E, G, I, K, M) and ppp1r14al MO-injected (B, D, F, H, J, L, N) embryos were assayed for expression of fgf3 at 10.5hpf (A, B), DUSP2 at 10.5hpf (C, D), hoxb1a at 11.5hpf (E, F) krox20 at 10.5hpf (G, H) and at 12hpf (I, J), pea3 at 12hpf (K, L) and valentino at 12.5hpf (M, N). (O–R) Analysis of fgf3 expression in control (O), ppp1r14al MO-injected (P), ppp1r14al MO + ppp1r14al mRNA injected (Q; note that mRNA is mutated to resist MO effect) reveals rescue of the ppp1r14al MO phenotype at 10.5hpf. Actual data is given in R. Phenotypes were scored as severe (similar to panel P), or mild (similar to panel B). Embryos in A–Q are shown as flat mounts in dorsal view with anterior to the top.

Expression Data

Genes:	dusp2 egr2b etv4 fgf3 hoxb1a mafba
Fish:	WT WT + MO1-ppp1r14ab
Knockdown Reagent:	MO1-ppp1r14ab
Anatomical Terms:	hindbrain neural rod midbrain hindbrain boundary neural rod presumptive rhombomere 3 presumptive rhombomere 4 presumptive rhombomere 5 presumptive rhombomere 6
Stage Range:	1-4 somites to 5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 358(2), Choe, S.K., Zhang, X., Hirsch, N., Straubhaar, J., and Sagerström, C.G., A screen for hoxb1-regulated genes identifies ppp1r14al as a regulator of the rhombomere 4 Fgf-signaling center, 356-67, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.