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ZFIN ID: ZDB-FIG-110811-24
Wingert et al., 2011 - Zebrafish nephrogenesis involves dynamic spatiotemporal expression changes in renal progenitors and essential signals from retinoic acid and irx3b. Developmental dynamics : an official publication of the American Association of Anatomists   240(8):2011-2027 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: 26+ somites
PHENOTYPE:
Fish:
Observed In:
Stage: 26+ somites

Fig. 5 Genetic and chemical models of retinoic acid (RA) biosynthesis deficiency exhibit a similar nephron segment phenotype of reduced proximal fates and expanded distal fates, and lib is the most severely affected aldh1a2 mutant. Whole-mount in situ hybridization analysis for nephron segment markers (purple) and mhc (red) at the 28 somite stage in aldh1a2 mutants and diaminobenzaldehyde (DEAB) -treated wild-type embryos. Embryos are shown in lateral views with anterior to the left, with the exception of dorsal views to show podocytes. Black brackets and arrows indicated expression domains, and numbers correspond to somite position. A,B: Proximal fates of podocytes, the proximal convoluted tubule (PCT) and proximal straight tubule (PST), were all reduced in the absence of RA biosynthesis (A), whereas distal fates of the distal early (DE), distal late (DL), corpuscle of Stannius (CS), and pronephric duct (PD) were expanded (B). C: Summary of nephron segmentation with respect to embryo somite number. Each color represents a different epithelial population: podocytes (green), PCT (orange), PST (yellow), DE (light blue), CS (red lettering indicates location among nephron tubule cells), DL (dark blue), and PD (black), and the overlap between DL and PD-expressed genes is indicated in white. The nephron segment phenotypes among RA-deficient zebrafish embryos showed a graded severity such that: nls homozygote < nls/lib compound heterozygotes < lib homozygotes < DEAB-treated wild-type embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gata3 TU standard conditions 26+ somites corpuscles of Stannius ISH
26+ somites posterior pronephric duct ISH
aldh1a2i26/+; aldh1a2nz15/+(TU) standard conditions 26+ somites corpuscles of Stannius ISH
26+ somites posterior pronephric duct ISH
aldh1a2i26/i26 standard conditions 26+ somites corpuscles of Stannius ISH
26+ somites posterior pronephric duct ISH
aldh1a2nz15/nz15(TU) standard conditions 26+ somites corpuscles of Stannius ISH
26+ somites posterior pronephric duct ISH
slc12a1 TU standard conditions 26+ somites pronephric distal early tubule ISH
aldh1a2i26/+; aldh1a2nz15/+(TU) standard conditions 26+ somites pronephric distal early tubule ISH
aldh1a2i26/i26 standard conditions 26+ somites pronephric distal early tubule ISH
aldh1a2nz15/nz15(TU) standard conditions 26+ somites pronephric distal early tubule ISH
slc12a3 TU standard conditions 26+ somites pronephric distal late tubule ISH
aldh1a2i26/+; aldh1a2nz15/+(TU) standard conditions 26+ somites pronephric distal late tubule ISH
aldh1a2i26/i26 standard conditions 26+ somites pronephric distal late tubule ISH
aldh1a2nz15/nz15(TU) standard conditions 26+ somites pronephric distal late tubule ISH
slc20a1a TU standard conditions 26+ somites pronephric proximal convoluted tubule ISH
aldh1a2i26/+; aldh1a2nz15/+(TU) standard conditions 26+ somites pronephric proximal convoluted tubule ISH
aldh1a2i26/i26 standard conditions 26+ somites pronephric proximal convoluted tubule ISH
aldh1a2nz15/nz15(TU) standard conditions 26+ somites pronephric proximal convoluted tubule ISH
trpm7 TU standard conditions 26+ somites pronephric proximal straight tubule ISH
aldh1a2i26/+; aldh1a2nz15/+(TU) standard conditions 26+ somites pronephric proximal straight tubule ISH
aldh1a2i26/i26 standard conditions 26+ somites pronephric proximal straight tubule ISH
aldh1a2nz15/nz15(TU) standard conditions 26+ somites pronephric proximal straight tubule ISH
wt1b TU standard conditions 26+ somites podocyte ISH
aldh1a2i26/+; aldh1a2nz15/+(TU) standard conditions 26+ somites podocyte ISH
aldh1a2i26/i26 standard conditions 26+ somites podocyte ISH
aldh1a2nz15/nz15(TU) standard conditions 26+ somites podocyte ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
aldh1a2i26/+; aldh1a2nz15/+(TU) standard conditions 26+ somites pronephric duct distal region increased length, abnormal
26+ somites pronephric duct proximal region decreased length, abnormal
aldh1a2i26/i26 standard conditions 26+ somites pronephric duct distal region increased length, abnormal
26+ somites pronephric duct proximal region decreased length, abnormal
aldh1a2nz15/nz15(TU) standard conditions 26+ somites pronephric duct distal region increased length, abnormal
26+ somites pronephric duct proximal region decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.