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ZFIN ID: ZDB-FIG-110811-23
Wingert et al., 2011 - Zebrafish nephrogenesis involves dynamic spatiotemporal expression changes in renal progenitors and essential signals from retinoic acid and irx3b. Developmental dynamics : an official publication of the American Association of Anatomists   240(8):2011-2027 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: 20-25 somites
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 4 lib is the most severe genetic model of aldh1a2-deficiency in the zebrafish. A: Lateral views of living 24 and 48 hours postfertilization (hpf) wild-type embryos, nls homozygotes, nls/lib compound heterozygotes, lib homozygotes, and diaminobenzaldehyde (DEAB) -treated wild-types. aldh1a2 mutations are associated with a truncation of the cervical region evident at 24 hpf (arrows indicate the caudal boundary of the otic vesicle [OV] and the rostral boundary of the first somite [S1]). DEAB-treated embryos lack this region, with the OV and S1 located adjacent to each other (indicated by single arrow). At 48 hpf, aldh1a2 mutations are associated with a kink at the head-trunk boundary (black arrow) and pericardial edema (*). Also at 48 hpf, lib and DEAB-treated embryos show pronounced body curvature and develop a lightbulb-shaped yolk compared with wild-types and other aldh1a2 mutants. B: Meiotic mapping placed lib in a genetic interval on linkage group (LG) 7 between z8693 and z11894 that includes aldh1a2. C: Reverse transcriptase-polymerase chain reaction and sequence analysis of aldh1a2 transcripts from lib mutants detected a C → A transversion at nucleotide 174 that is predicted to introduce a Stop codon in lieu of a Tyrosine residue at amino acid position 58 in the 519 amino acid raldh2 enzyme. D: Whole-mount in situ hybridization analysis of aldh1a2 expression (purple) and krox20 expression (red) shows decreased aldh1a2 transcripts both in lib mutants and lib heterozygote embryos, consistent with nonsense mediated decay.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
aldh1a2 WT standard conditions 20-25 somites pronephric duct ISH
20-25 somites segmental plate ISH
20-25 somites somite ISH
aldh1a2nz15/nz15(TU) standard conditions 20-25 somites pronephric duct ISH
20-25 somites segmental plate ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
aldh1a2i26/+; aldh1a2nz15/+(TU) standard conditions Prim-5 otic vesicle decreased distance somite 1, abnormal
Prim-5 vertebral column anterior region decreased length, abnormal
Long-pec head misaligned with trunk, abnormal
Long-pec pericardium edematous, abnormal
aldh1a2i26/i26 standard conditions Prim-5 otic vesicle decreased distance somite 1, abnormal
Prim-5 vertebral column anterior region decreased length, abnormal
Long-pec head misaligned with trunk, abnormal
Long-pec pericardium edematous, abnormal
aldh1a2nz15/nz15(TU) standard conditions Prim-5 otic vesicle decreased distance somite 1, abnormal
Prim-5 vertebral column anterior region decreased length, abnormal
Long-pec head misaligned with trunk, abnormal
Long-pec pericardium edematous, abnormal
Long-pec post-vent region curved ventral, abnormal
Long-pec yolk deformed, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.