ZFIN ID: ZDB-FIG-110622-87
Yang et al., 2011 - BMP and non-canonical Wnt signaling are required for inhibition of secondary tail formation in zebrafish. Development (Cambridge, England)   138(12):2601-2611 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Fig. 3

Non-canonical Wnt signaling functions with BMP to inhibit secondary tail formation. (A-C) Lateral view of wild-type (A, 24 hpf), dvl2/3 morphant (B, 24 hpf) and kny mutant (C, 72 hpf) zebrafish. Insets of A and B show ntl expression in the posterior tail. Inset of C shows a close-up of posterior tail. Primary and secondary tails are marked with arrowheads. (D-I) Lateral view of expression of ntl (D,E), col2a (col2α F,G) and myoD (H,I) in the tail of wild-type and kny embryos fixed at 24 hpf. ntl (E), col2a (G), and myoD (I) exhibit ectopic expression within the tails of kny. (J) Percentages of secondary tail formation in embryos injected with indicated combination of morpholinos (MO; 1 mg/ml each, except for ‘dvl2/dvl3 MO’ condition, where a concentration of 3 mg/ml for each MO was injected). For each column over 100 embryos from three separate experiments were scored. (K) Percentages of secondary tail formation in embryos from incross of mfn/+;kny/+ double mutant carriers. Embryos are categorized by phenotypes. Secondary tails in J and K were scored by ntl expression.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
col2a1a WT standard conditions Prim-5 notochord ISH
gpc4fr6/fr6 standard conditions Prim-5 notochord ISH
myod1 WT standard conditions Prim-5 muscle ISH
gpc4fr6/fr6 standard conditions Prim-5 muscle ISH
tbxta WT standard conditions Prim-5 notochord ISH
Prim-5 tail bud ISH
gpc4fr6/fr6 standard conditions Prim-5 notochord ISH
Prim-5 tail bud ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-dvl2 + MO1-dvl3a standard conditions Prim-5 post-anal tail morphogenesis disrupted, abnormal
Prim-5 post-anal tail morphogenesis having extra processual parts post-anal tail morphogenesis, abnormal
Prim-5 post-vent region deformed, abnormal
Prim-5 whole organism has extra parts of type post-vent region, abnormal
WT + MO1-dvl2 + MO1-dvl3a + MO2-tll1 standard conditions Prim-5 post-anal tail morphogenesis disrupted, abnormal
Prim-5 post-anal tail morphogenesis having extra processual parts post-anal tail morphogenesis, abnormal
Prim-5 post-vent region deformed, abnormal
Prim-5 whole organism has extra parts of type post-vent region, abnormal
WT + MO1-dvl2 + MO2-tll1 standard conditions Prim-5 post-anal tail morphogenesis disrupted, abnormal
Prim-5 post-anal tail morphogenesis having extra processual parts post-anal tail morphogenesis, abnormal
Prim-5 post-vent region deformed, abnormal
Prim-5 whole organism has extra parts of type post-vent region, abnormal
WT + MO1-dvl3a + MO2-tll1 standard conditions Prim-5 post-anal tail morphogenesis disrupted, abnormal
Prim-5 post-anal tail morphogenesis having extra processual parts post-anal tail morphogenesis, abnormal
Prim-5 post-vent region deformed, abnormal
Prim-5 whole organism has extra parts of type post-vent region, abnormal
WT + MO2-dvl1b + MO2-tll1 standard conditions Prim-5 post-anal tail morphogenesis disrupted, abnormal
Prim-5 post-anal tail morphogenesis having extra processual parts post-anal tail morphogenesis, abnormal
Prim-5 post-vent region deformed, abnormal
Prim-5 whole organism has extra parts of type post-vent region, abnormal
WT + MO2-tll1 standard conditions Prim-5 post-anal tail morphogenesis disrupted, abnormal
Prim-5 post-anal tail morphogenesis having extra processual parts post-anal tail morphogenesis, abnormal
Prim-5 post-vent region deformed, abnormal
Prim-5 whole organism has extra parts of type post-vent region, abnormal
gpc4fr6/+; tll1tc263a/tc263a standard conditions Prim-5 post-anal tail morphogenesis disrupted, abnormal
Prim-5 post-anal tail morphogenesis having extra processual parts post-anal tail morphogenesis, abnormal
Prim-5 post-vent region deformed, abnormal
Prim-5 whole organism has extra parts of type post-vent region, abnormal
gpc4fr6/fr6 standard conditions Prim-5 post-anal tail morphogenesis disrupted, abnormal
Prim-5 post-anal tail morphogenesis having extra processual parts post-anal tail morphogenesis, abnormal
Prim-5 post-vent region deformed, abnormal
Prim-5 whole organism has extra parts of type post-vent region, abnormal
Protruding-mouth post-anal tail morphogenesis disrupted, abnormal
Protruding-mouth post-anal tail morphogenesis having extra processual parts post-anal tail morphogenesis, abnormal
Protruding-mouth post-vent region deformed, abnormal
Protruding-mouth whole organism has extra parts of type post-vent region, abnormal
gpc4fr6/fr6; tll1tc263a/+ standard conditions Prim-5 post-anal tail morphogenesis disrupted, abnormal
Prim-5 post-anal tail morphogenesis having extra processual parts post-anal tail morphogenesis, abnormal
Prim-5 post-vent region deformed, abnormal
Prim-5 whole organism has extra parts of type post-vent region, abnormal
gpc4fr6/fr6; tll1tc263a/tc263a standard conditions Prim-5 post-anal tail morphogenesis disrupted, abnormal
Prim-5 post-anal tail morphogenesis having extra processual parts post-anal tail morphogenesis, abnormal
Prim-5 post-vent region deformed, abnormal
Prim-5 whole organism has extra parts of type post-vent region, abnormal
tll1tc263a/tc263a control Prim-5 post-anal tail morphogenesis disrupted, abnormal
Prim-5 post-anal tail morphogenesis having extra processual parts post-anal tail morphogenesis, abnormal
Prim-5 post-vent region deformed, abnormal
Prim-5 whole organism has extra parts of type post-vent region, abnormal
tll1tc263a/tc263a + MO1-dvl2 + MO1-dvl3a standard conditions Prim-5 post-anal tail morphogenesis disrupted, abnormal
Prim-5 post-anal tail morphogenesis having extra processual parts post-anal tail morphogenesis, abnormal
Prim-5 post-vent region deformed, abnormal
Prim-5 whole organism has extra parts of type post-vent region, abnormal
Acknowledgments:
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