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ZFIN ID: ZDB-FIG-110622-86
Yang et al., 2011 - BMP and non-canonical Wnt signaling are required for inhibition of secondary tail formation in zebrafish. Development (Cambridge, England)   138(12):2601-2611 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: 5-9 somites to Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-5

Fig. 2

Secondary tail formation is independent of the role of BMP signaling in fate patterning. (A-E) Lateral view of zebrafish tails in wild-type and dorsalized embryos of indicated genotypes at 24 hpf, after whole-mount in situ for ntl. Primary and secondary tails are marked with arrowheads. C1-C4 refer to the degree of dorsalization using the dorso-anterior index. (F) Distribution of secondary tail phenotype in embryos following dorsomorphin (DM) treatment at the indicated time points. Secondary tails were scored by the presence of ectopic ntl expression. (G) Lateral view of the tail of a live embryo at 24 hpf, after DM treatment at 7 somites (7s). Note the secondary tail with fully developed ventral fin. (H-M) Dorsal view of embryos expressing gata1 (H,I), fli1 (J,K) at the 13-somite stage and myoD (L,M) at 24 hpf in the tail, after treatment with DMSO (H,J,L) and DM (I,K,M) at the 5-somite stage. (N,O) Dorsal view of myoD expression in the tail of wild-type (wt; J) and mfn (K) embryos at 22 hpf. Inset shows the lateral view. Expression of gata1 (I), fli1 (K) and myoD (M,O) is unaffected.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
fli1 WT control 5-9 somites posterior lateral mesoderm ISH
WT chemical treatment: dorsomorphin 5-9 somites posterior lateral mesoderm ISH
gata1a WT control 5-9 somites posterior lateral plate mesoderm ISH
WT chemical treatment: dorsomorphin 5-9 somites posterior lateral plate mesoderm ISH
myod1 WT control 26+ somites muscle ISH
WT chemical treatment: dorsomorphin 26+ somites muscle ISH
tll1tc263a/tc263a control 26+ somites muscle ISH
tbxta WT standard conditions Prim-5 notochord ISH
Prim-5 tail bud ISH
bmp7aty68a/ty68a standard conditions Prim-5 notochord ISH
Prim-5 tail bud ISH
smad5dty40/+ standard conditions Prim-5 notochord ISH
Prim-5 tail bud ISH
smad5dty40/dty40 standard conditions Prim-5 notochord ISH
Prim-5 tail bud ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
bmp7aty68a/ty68a standard conditions Prim-5 post-anal tail morphogenesis disrupted, abnormal
Prim-5 post-anal tail morphogenesis having extra processual parts post-anal tail morphogenesis, abnormal
Prim-5 post-vent region has extra parts of type notochord, abnormal
Prim-5 post-vent region has extra parts of type tail bud, abnormal
Prim-5 whole organism has extra parts of type post-vent region, abnormal
Prim-5 whole organism wholly dorsalized, abnormal
smad5dty40/+ standard conditions Prim-5 post-anal tail morphogenesis disrupted, abnormal
Prim-5 post-anal tail morphogenesis having extra processual parts post-anal tail morphogenesis, abnormal
Prim-5 post-vent region has extra parts of type notochord, abnormal
Prim-5 post-vent region has extra parts of type tail bud, abnormal
Prim-5 whole organism has extra parts of type post-vent region, abnormal
Prim-5 whole organism wholly dorsalized, abnormal
smad5dty40/dty40 standard conditions Prim-5 post-anal tail morphogenesis disrupted, abnormal
Prim-5 post-anal tail morphogenesis having extra processual parts post-anal tail morphogenesis, abnormal
Prim-5 post-vent region has extra parts of type notochord, abnormal
Prim-5 post-vent region has extra parts of type tail bud, abnormal
Prim-5 whole organism has extra parts of type post-vent region, abnormal
Prim-5 whole organism wholly dorsalized, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development