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ZIRC
ZFIN ID: ZDB-FIG-110622-90
Yang et al., 2011 - BMP and non-canonical Wnt signaling are required for inhibition of secondary tail formation in zebrafish. Development (Cambridge, England)   138(12):2601-2611 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: 5-9 somites to 14-19 somites
PHENOTYPE:
Fish:
Observed In:
Stage Range: 10-13 somites to 14-19 somites

Fig. 6

Defects of the caudal end of notochord in mfn and kny initiate during tailbud protrusion. (A) Dorsal view of flat-mounted tailbud of a 6-somite wild-type zebrafish embryo, after fluorescence in situ hybridization (FISH) for flh (red) and antibody staining for P-Samd5 (green); no colocalization (merge) is seen. (B,C) Lateral view of tails of 14-somite wild-type and mfn embryos exposed to P-Smad5 (green) and β-catenin (red) antibodies. P-Smad5 staining is absent in the posterior tailbud of mfn (C, merge). Arrowheads indicate the ventroposterior cells in the tailbud that are normally positive for P-Smad5 (B, merge). (D-F4) Confocal time-lapse recording of tailbuds in embryos expressing Tg(flh:EGFP) transgene (green) and mRFP (red) from the 10-somite stage. Shown are wild type (D-D4) and mfn (E-E4) and kny (F-F4) mutants. Note that transgenic EGFP is retained in the caudal notochord, floorplate and periphery of Kupffer′s vesicle (KV; marked by the white line). The caudal end of notochord (CN) is indicated by blue line. Arrowheads mark the ventroposterior cells in the tailbud. Embryos were all mounted laterally.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP c161Tg standard conditions 10-13 somites tail bud IFL
gpc4fr6/+; c161Tg standard conditions 10-13 somites tail bud IFL
tll1tc263a/+; c161Tg standard conditions 10-13 somites tail bud IFL
noto WT standard conditions 5-9 somites posterior neural plate ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-smad IHC WT standard conditions 5-9 somites tail bud
IHC 14-19 somites tail bud
IHC tll1tc263a/tc263a standard conditions 14-19 somites tail bud
Ab2-ctnnb IHC WT standard conditions 14-19 somites tail bud
IHC tll1tc263a/tc263a standard conditions 14-19 somites tail bud
Phenotype Details
Fish Conditions Stage Phenotype
c161Tg standard conditions 10-13 somites post-vent region morphology, normal
gpc4fr6/+; c161Tg standard conditions 10-13 somites notochord formation disrupted, abnormal
10-13 somites post-anal tail morphogenesis disrupted, abnormal
10-13 somites post-anal tail morphogenesis having extra processual parts post-anal tail morphogenesis, abnormal
tll1tc263a/+; c161Tg standard conditions 10-13 somites notochord formation disrupted, abnormal
10-13 somites post-anal tail morphogenesis disrupted, abnormal
10-13 somites post-anal tail morphogenesis having extra processual parts post-anal tail morphogenesis, abnormal
tll1tc263a/tc263a standard conditions 14-19 somites notochord formation disrupted, abnormal
14-19 somites post-anal tail morphogenesis disrupted, abnormal
14-19 somites post-anal tail morphogenesis having extra processual parts post-anal tail morphogenesis, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development