ZFIN ID: ZDB-FIG-110408-4
Corti et al., 2011 - Interaction between alk1 and blood flow in the development of arteriovenous malformations. Development (Cambridge, England)   138(8):1573-1582 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Prim-25

Fig. 4

Loss of alk1 dysregulates expression of flow-responsive genes but does not alter shear stress. (A) Whole-mount in situ hybridization using klf2a, edn1 and cxcr4a riboprobes. Vascular klf2a expression is strongly downregulated in the absence of blood flow (tnnt2a-/- or tricaine treatment, 32-40 hpf) but is unaltered in alk1-/- embryos. By contrast, vascular edn1 expression is downregulated and cxcr4a expression is upregulated both in the absence of flow and in alk1 mutants. Changes in expression are primarily in alk1-positive arteries: ICA (blue arrow) and CaDI (white arrow), BCA (white arrowhead) and LDA (blue arrowhead), as well as AA1 (not shown). Lateral expression of both edn1 and cxcr4a is in the pharyngeal arches and is unchanged in all conditions. All embryos are 36 hpf except tricaine treated, which are 40 hpf. klf2a and edn1, lateral views, anterior leftwards. cxcr4a, dorsal view, anterior leftwards. Scale bar: 100 μm. (B) Blood flow in the distal region of AA1 was imaged in chrna1-/- (paralyzed) Tg(kdrl:GFP)la116;Tg(gata1:dsRed)sd2 alk1 mutant embryos and wild-type/heterozygous siblings at 32-36 hpf by high-speed confocal microscopy, and particle image velocimetry used to calculate wall shear stress. Results represent meanĀ±s.d., n=8 wild type and n=5 alk1 mutants. Differences were not significant according to Student′s t-test.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cxcr4a WT standard conditions Prim-25 basal communicating artery ISH
Prim-25 lateral dorsal aorta ISH
Prim-25 pharyngeal arch ISH
WT chemical treatment: pharmaceutical Prim-25 basal communicating artery ISH
Prim-25 lateral dorsal aorta ISH
Prim-25 pharyngeal arch ISH
acvrl1y6/y6 standard conditions Prim-25 basal communicating artery ISH
Prim-25 lateral dorsal aorta ISH
Prim-25 pharyngeal arch ISH
tnnt2atc300b/tc300b standard conditions Prim-25 basal communicating artery ISH
Prim-25 lateral dorsal aorta ISH
Prim-25 pharyngeal arch ISH
edn1 WT standard conditions Prim-25 aortic arch 1 ISH
Prim-25 caudal division of the internal carotid artery ISH
Prim-25 internal carotid artery ISH
Prim-25 lateral dorsal aorta ISH
Prim-25 pharyngeal arch ISH
WT chemical treatment: pharmaceutical Prim-25 Not Detected aortic arch 1 ISH
Prim-25 Not Detected caudal division of the internal carotid artery ISH
Prim-25 Not Detected internal carotid artery ISH
Prim-25 Not Detected lateral dorsal aorta ISH
Prim-25 pharyngeal arch ISH
acvrl1y6/y6 standard conditions Prim-25 Not Detected aortic arch 1 ISH
Prim-25 Not Detected caudal division of the internal carotid artery ISH
Prim-25 Not Detected internal carotid artery ISH
Prim-25 Not Detected lateral dorsal aorta ISH
Prim-25 pharyngeal arch ISH
tnnt2atc300b/tc300b standard conditions Prim-25 Not Detected aortic arch 1 ISH
Prim-25 Not Detected caudal division of the internal carotid artery ISH
Prim-25 Not Detected internal carotid artery ISH
Prim-25 Not Detected lateral dorsal aorta ISH
Prim-25 pharyngeal arch ISH
klf2a WT standard conditions Prim-25 vasculature ISH
WT chemical treatment: pharmaceutical Prim-25 vasculature ISH
acvrl1y6/y6 standard conditions Prim-25 vasculature ISH
tnnt2atc300b/tc300b standard conditions Prim-25 vasculature ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development