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ZFIN ID: ZDB-FIG-110408-2
Corti et al., 2011 - Interaction between alk1 and blood flow in the development of arteriovenous malformations. Development (Cambridge, England)   138(8):1573-1582 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-15 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 2

Retention of normally transient arteriovenous connections in alk1 mutants is flow dependent. (A) In wild-type embryos (row 1), transient connections between the basal communicating artery (BCA) and primordial midbrain channel (PMBC) carry blood at 32 hpf but regress by 48 hpf (arrows). In alk1 mutants (row two), one or both of these bilateral connections may be retained, forming a BCA-to-PMBC AVM (arrows). More posteriorly, lumenized connections drain the basilar artery (BA) to the primordial hindbrain channel (PHBC) in wild-type embryos at early times, but almost all regress by 48 hpf (row 3, arrows). In alk1 mutants, one or more of these connections may be retained, forming a BA-to-PHBC AVM (row 4, arrows). Images are two-dimensional confocal projections of Tg(kdrl:GFP)la116; Tg(gata1:dsRed)sd2 embryos, dorsal views, anterior leftwards. Endothelial cells are green; erythrocytes are magenta. (B) AVMs (arrows) are detectable in 48 hpf alk1-/- embryos and in alk1-/-;gata1a-/- embryos (which lack erythrocytes), but not in alk1-/-;tnnt2 MO (which lack blood flow). Images are two-dimensional confocal projections of Tg(fli1a.ep:mRFP-F)pt505 embryos, dorsal views, anterior leftwards. Scale bars: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed acvrl1y6/y6; la116Tg; sd2Tg standard conditions Prim-15 nucleate erythrocyte IFL
Prim-25 nucleate erythrocyte IFL
Long-pec nucleate erythrocyte IFL
la116Tg; sd2Tg standard conditions Prim-15 nucleate erythrocyte IFL
Prim-25 nucleate erythrocyte IFL
Long-pec nucleate erythrocyte IFL
EGFP acvrl1y6/y6; la116Tg; sd2Tg standard conditions Prim-15 endothelial cell IFL
Prim-25 endothelial cell IFL
Long-pec endothelial cell IFL
la116Tg; sd2Tg standard conditions Prim-15 endothelial cell IFL
Prim-25 endothelial cell IFL
Long-pec endothelial cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
acvrl1y6/y6 + MO1-tnnt2a standard conditions Long-pec basal communicating artery detached from primordial midbrain channel, normal
Long-pec basilar artery detached from primordial hindbrain channel, normal
Long-pec blood circulation absent, abnormal
Long-pec heart contraction absent, abnormal
acvrl1y6/y6; gata1am651/m651 standard conditions Long-pec basal communicating artery attached to primordial midbrain channel, abnormal
Long-pec basilar artery attached to primordial hindbrain channel, abnormal
Long-pec nucleate erythrocyte decreased amount, abnormal
acvrl1y6/y6; la116Tg; sd2Tg standard conditions Long-pec basal communicating artery attached to primordial midbrain channel, abnormal
Long-pec basilar artery attached to primordial hindbrain channel, abnormal
Long-pec blood vessel remodeling decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development